Palermo M S, Olabuenaga S E, Giordano M, Isturiz M A
Int J Immunopharmacol. 1986;8(6):651-5. doi: 10.1016/0192-0561(86)90038-x.
Metabolism of cyclophosphamide (Cy) by liver enzymes results in cytostatic products and acrolein, which exerts urotoxicity. Experiments were designed to determine which metabolites are responsible for Cy-induced immunomodulation. For this purpose, mice were treated simultaneously with Cy and N-acetyl-cysteine (NAC), a thiol compound which reacts with acrolein, and different immunological functions were assayed. Results show that NAC did not interfere with Cy effects on antibody-dependent cellular cytotoxicity (ADCC), NK activity, delayed-type hypersensitivity (DTH) or antibody production, indicating that modulation of these functions by Cy is mediated by its cytostatic metabolites.
肝脏酶对环磷酰胺（Cy）的代谢会产生细胞抑制产物和丙烯醛，后者具有尿毒性。设计实验以确定哪些代谢产物导致Cy诱导的免疫调节。为此，将小鼠同时用Cy和N-乙酰半胱氨酸（NAC，一种与丙烯醛反应的硫醇化合物）处理，并检测不同的免疫功能。结果表明，NAC不干扰Cy对抗体依赖性细胞毒性（ADCC）、自然杀伤细胞（NK）活性、迟发型超敏反应（DTH）或抗体产生的影响，这表明Cy对这些功能的调节是由其细胞抑制性代谢产物介导的。
