Saijo N, Irimajiri N, Ozaki A, Takizawa T, Beppu Y, Shibuya M, Shimizu E
Gan. 1982 Apr;73(2):270-7.
The effects of BCG and cyclophosphamide on the antibody-dependent cell-mediated cytotoxicity (ADCC) and natural killer (NK) activity of spleen and peritoneal lymphocytes were serially examined after treatment. The changes of ADCC and NK activity were different in peritoneal and spleen lymphocytes according to the route and timing of administration. The decrease of NK activity induced by cyclophosphamide is not marked, and the degree of decrease depends on the timing of cyclophosphamide administration. The ADCC and NK activity of peritoneal lymphocytes after intravenous BCG administration were elevated 7 days after treatment, but the NK activity of spleen lymphocytes was decreased. On the other hand, the NK activities of peritoneal and spleen lymphocytes were elevated in rats given intraperitoneal BCG. Spleen lymphocytes and peritoneal lymphocytes from rats given intravenous BCG suppressed tumor growth in the Winn test during the early tumor-bearing stage. The spleen lymphocytes with low NK activity in rats given intravenous BCG strongly suppressed the NK activity of normal spleen lymphocytes.
治疗后连续检测卡介苗（BCG）和环磷酰胺对脾脏及腹腔淋巴细胞的抗体依赖性细胞介导的细胞毒性（ADCC）和自然杀伤（NK）活性的影响。根据给药途径和时间，腹腔和脾脏淋巴细胞中ADCC和NK活性的变化有所不同。环磷酰胺诱导的NK活性降低并不明显，降低程度取决于环磷酰胺的给药时间。静脉注射卡介苗后7天，腹腔淋巴细胞的ADCC和NK活性升高，但脾脏淋巴细胞的NK活性降低。另一方面，腹腔注射卡介苗的大鼠腹腔和脾脏淋巴细胞的NK活性升高。在早期荷瘤阶段，静脉注射卡介苗的大鼠的脾脏淋巴细胞和腹腔淋巴细胞在Winn试验中抑制肿瘤生长。静脉注射卡介苗的大鼠中NK活性低的脾脏淋巴细胞强烈抑制正常脾脏淋巴细胞的NK活性。
