College of Science, Sichuan Agricultural University, Xin Kang Road,Yucheng District, Ya'an 625014, P. R. China.
College of Information Engineering, Sichuan Agricultural University, Xin Kang Road,Yucheng District, Ya'an 625014, P. R. China.
ACS Appl Mater Interfaces. 2023 Nov 29;15(47):54466-54477. doi: 10.1021/acsami.3c12157. Epub 2023 Nov 16.
In this work, CuO/FeO nanozymes with high peroxidase-like activity were synthesized by using hydrothermal and calcination methods. The high-resolution transmission electron microscopy (HRTEM) proved that the heterogeneous interface of CuO/FeO was the main reason for the high enzyme-like activity. Strong interactions of CuO and FeO were successfully verified by X-ray absorption near-edge structure (XANES) characterization. Experiments and density functional theory (DFT) calculations were also used to explain the increased enzyme activity. The heterogeneous interface acted as the main active center, facilitating the electron transfer from CuO to FeO. A colorimetric and intelligent sensing system was constructed based on deep learning. Using the peroxidase-like activity of CuO/FeO, a platform for glufosinate pesticides and chlortetracycline hydrochloride (CTC) with the signal "on-off-on" changes were established. The limit of detection (LOD) of glufosinate and CTC was 28 and 0.69 μM, respectively. It was successfully applied in the detection of environmental water and soil. This study can provide an intelligent detection method for environmental monitoring.
在这项工作中，通过水热和煅烧方法合成了具有高过氧化物酶样活性的 CuO/FeO 纳米酶。高分辨率透射电子显微镜（HRTEM）证明，CuO/FeO 的异质界面是高酶样活性的主要原因。X 射线吸收近边结构（XANES）表征成功验证了 CuO 和 FeO 之间的强相互作用。实验和密度泛函理论（DFT）计算也用于解释酶活性的增加。异质界面充当主要的活性中心，促进了 CuO 向 FeO 的电子转移。基于深度学习构建了比色和智能传感系统。利用 CuO/FeO 的过氧化物酶样活性，建立了具有“开-关-开”信号变化的草甘膦和盐酸金霉素（CTC）农药平台。草甘膦和 CTC 的检测限（LOD）分别为 28 和 0.69 μM。它已成功应用于环境水和土壤的检测。本研究可为环境监测提供一种智能检测方法。
