Ruiz María J, Sirini Noelí E, Stegmayer María Á, Soto Lorena P, Zbrun María V, Olivero Carolina R, Werning María L, Acosta Federico F, Signorini Marcelo L, Frizzo Laureano S
Laboratory of Food Analysis "Rodolfo Oscar Dalla Santina", Institute of Veterinary Science (ICiVet Litoral), National University of the Litoral-National Council of Scientific and Technical Research (UNL/CONICET), Esperanza, Province of Santa Fe S3080, Argentina.
Department of Animal Health and Preventive Medicine, Faculty of Veterinary Sciences, National University of the Centre of the Province of Buenos Aires, Tandil, Buenos Aires 7000, Argentina.
J Appl Microbiol. 2023 Dec 1;134(12). doi: 10.1093/jambio/lxad267.
To evaluate the biofilm-forming capacity of thermotolerant Campylobacter (TC) strains from poultry production and to analyse the inhibitory capacity of Lactiplantibacillus plantarum LP5 against TC on different materials.
Biofilm-forming capacity by Campylobacter jejuni and Campylobacter coli was analysed by cell adhesion in polystyrene plates. TC were classified as non-biofilm-forming (NBF, 1.3%), weak biofilm-forming (WBF, 68.4%), moderate biofilm-forming (MBF, 27.6%), and strong biofilm-forming (SBF, 2.7%). The inhibitory capacity of L. plantarum LP5 against TC was tested on stainless-steel, nylon, aluminium, and glass disks (treated group) and compared with biofilm-forming TC (control group). Lactiplantibacillus plantarum LP5 was inoculated, and then TC. Biofilm was removed in both experimental groups and TC and LP5 bacterial counts were performed. The L. plantarum LP5 presence reduced the formation of TC biofilm (P < 0.001). The material type and strain category influenced biofilm formation, with stainless-steel and the SBF strain being the material and TC having the highest adhesion (P < 0.001). Lactiplantibacillus plantarum LP5 formed a similar biofilm on all materials (P = 0.823).
This trial showed very promising results; L. plantarum LP5 could be incorporated as a bio-protector of TC on different surfaces.
评估来自家禽生产的耐热弯曲杆菌（TC）菌株的生物膜形成能力，并分析植物乳杆菌LP5对不同材料上的TC的抑制能力。
通过空肠弯曲杆菌和结肠弯曲杆菌在聚苯乙烯平板上的细胞黏附来分析生物膜形成能力。TC被分类为非生物膜形成（NBF，1.3%）、弱生物膜形成（WBF，68.4%）、中度生物膜形成（MBF，27.6%）和强生物膜形成（SBF，2.7%）。在不锈钢、尼龙、铝和玻璃圆盘上测试植物乳杆菌LP5对TC的抑制能力（处理组），并与形成生物膜的TC（对照组）进行比较。接种植物乳杆菌LP5，然后接种TC。在两个实验组中去除生物膜，并进行TC和LP5细菌计数。植物乳杆菌LP5的存在减少了TC生物膜的形成（P < 0.001）。材料类型和菌株类别影响生物膜形成，不锈钢和SBF菌株分别是使TC具有最高黏附力的材料和菌株（P < 0.001）。植物乳杆菌LP5在所有材料上形成的生物膜相似（P = 0.823）。
该试验显示出非常有前景的结果；植物乳杆菌LP5可作为不同表面上TC的生物保护剂。
