Laboratory of Food Analysis 'Rodolfo Oscar Dalla Santina', Institute of Veterinary Science (ICiVet Litoral), National University of the Litoral - National Council of Scientific and Technical Research (UNL/CONICET), Kreder 2805, 3080 Esperanza, Province of Santa Fe, Argentina.
Department of Animal Health and Preventive Medicine, Faculty of Veterinary Sciences, National University of the Center of the Province of Buenos Aires, Tandil, Argentina.
Benef Microbes. 2021 Nov 16;12(6):553-565. doi: 10.3920/BM2021.0010. Epub 2021 Sep 30.
Thermotolerant species are the leading cause of foodborne bacterial diarrheal disease worldwide. , abundant in pigs and pork products, have been identified as a source of human infection. In this study, we propose the use of LP5 as a probiotic to reduce colonisation of this intestinal pathogen in a murine colonisation model of DSPV458. Six-week-old adult female mice were housed in groups: Control, Campy and Pro-Campy. Control and Pro-Campy groups received antibiotics for 5 days and the Campy group for 12 days. Pro-Campy group was inoculated for 7 days with 8.78 log cfu total of LP5 suspended in De Man, Rogosa and Sharpe broth. All groups were inoculated with 6.72 log cfu of DSPV458 suspended in brain heart infusion broth. LP5 was recovered only in the Pro- Campy group. DSPV458 was recovered at higher levels in the Control and Campy groups. The differences with the Pro-Campy group were significant. As regards faeces, Control and Campy groups reached 7.41 and 7.84 log cfu/g, respectively, and the Pro-Campy group only 4.62 log cfu/g. In the caecum, Control and Campy groups reached 8.01 and 9.26 logcfu/g, respectively, and the Pro-Campy group only 4.51 log cfu/g. In the ileum, Control and Campy groups reached 3.43 and 3.26 log cfu/g, respectively, and the Pro-Campy group did not show detectable levels. The reduction of DSPV458 in the Pro-Campy group compared to the Control group in faeces, caecum and ileum was 99.55, 99.98 and 100%, respectively. Animals were maintained under normal health conditions, and haematological parameters were within the standard values for . The incorporation of a probiotic generated a protective effect in the mice colonisation model. The protective effect would also apply to intestinal colonisation by indigenous enterobacteria. Therefore, the strategy used in this study is of great importance to understand the protection mechanisms in a murine model, as well as its application in food-producing animals.
耐热物种是全球食源性细菌性腹泻病的主要原因。, 在猪和猪肉产品中大量存在，已被确定为人畜感染的来源。在本研究中，我们提出使用 LP5 作为益生菌来减少 DSPV458 小鼠定植模型中这种肠道病原体的定植。6 周龄成年雌性 小鼠分组饲养：对照组、Campy 组和 Pro-Campy 组。对照组和 Pro-Campy 组接受 5 天抗生素治疗，Campy 组接受 12 天抗生素治疗。Pro-Campy 组连续 7 天用 8.78 log cfu 总数的 LP5 悬浮于 De Man、Rogosa 和 Sharpe 肉汤中进行接种。所有组均用 6.72 log cfu 悬浮于脑心浸液肉汤中的 DSPV458 进行接种。LP5 仅在 Pro-Campy 组中回收。在对照和 Campy 组中，DSPV458 的回收率更高。与 Pro-Campy 组的差异具有统计学意义。关于粪便，对照组和 Campy 组分别达到 7.41 和 7.84 log cfu/g，而 Pro-Campy 组仅为 4.62 log cfu/g。在盲肠中，对照组和 Campy 组分别达到 8.01 和 9.26 log cfu/g，而 Pro-Campy 组仅为 4.51 log cfu/g。在回肠中，对照组和 Campy 组分别达到 3.43 和 3.26 log cfu/g，而 Pro-Campy 组未检测到。与对照组相比，Pro-Campy 组在粪便、盲肠和回肠中对 DSPV458 的减少分别为 99.55%、99.98%和 100%。动物保持在正常健康状态，血液学参数在 的标准值范围内。益生菌的加入对小鼠定植模型产生了保护作用。该保护作用也适用于土著肠杆菌对肠道的定植。因此，本研究中使用的策略对于了解小鼠模型中的保护机制及其在生产动物中的应用非常重要。
