Curukoglu Ali, Gungor Gul Ciray Akbas, Akan Gokce, Kukner Aysel, Ogutcu Gozde, Kalayci Melis, Temizel Meliha, Ozgencil Fatma Eser
Surgery Department, Faculty of Veterinary Medicine, Near East University, Yakin Dogu St, Nicosia, Mersin, Turkiye.
DESAM Institute, Near East University, Yakin Dogu St, Nicosia, Mersin, Turkiye.
Vet Med (Praha). 2023 Apr 28;68(4):152-163. doi: 10.17221/109/2022-VETMED. eCollection 2023 Apr.
This study was planned to investigate an alternative treatment modality in diabetic wound healing. In this experimental study, the efficacy of both cold atmospheric plasma/nitric oxide (NO) and NPH insulin ointment, recently known to have beneficial effects on wound healing, was investigated in diabetic wound healing. Twenty-four (24) diabetic rats were divided into four groups DC, DI, DNO and DINO (diabetic control, diabetic insulin, diabetic nitric oxide, diabetic insulin + nitric oxide groups). No treatment was applied to the DC group, NPH insulin was applied to the DI group, CAP/NO was applied to the DNO group, and CAP/NO + NPH insulin was applied to the DINO group once daily for 14 days. The wound area reduction and the wound contraction rate were calculated on the basis of the tissue sections taken, and histopathological and genetic analyses were carried out. Compared to the control group, exogenous NO gas was found to be a potent antibacterial agent in the diabetic wound healing, causing a reduction in the wound area ( = 0.034), an increased contraction rate ( = 0.021), epithelialisation ( = 0.02), collagen organisation ( = 0.006) and a reduction in the number of inflammatory cells ( = 0.002). A significant increase in the expression of IL-8 mRNA was observed ( = 0.026). It was concluded that NPH insulin alone contributes to wound healing, but it is not necessary to use it together with exogenous NO gas.
本研究旨在探讨糖尿病伤口愈合的一种替代治疗方式。在这项实验研究中，研究了冷大气等离子体/一氧化氮（NO）和NPH胰岛素软膏（最近已知对伤口愈合有有益作用）在糖尿病伤口愈合中的疗效。将24只糖尿病大鼠分为四组：DC、DI、DNO和DINO组（糖尿病对照组、糖尿病胰岛素组、糖尿病一氧化氮组、糖尿病胰岛素+一氧化氮组）。DC组不进行治疗，DI组应用NPH胰岛素，DNO组应用冷大气等离子体/一氧化氮，DINO组应用冷大气等离子体/一氧化氮+NPH胰岛素，每天一次，持续14天。根据所取组织切片计算伤口面积缩小率和伤口收缩率，并进行组织病理学和基因分析。与对照组相比，发现外源性NO气体在糖尿病伤口愈合中是一种有效的抗菌剂，可导致伤口面积减小（P = 0.034）、收缩率增加（P = 0.021）、上皮化（P = 0.02）、胶原组织形成（P = 0.006）以及炎症细胞数量减少（P = 0.002）。观察到IL-8 mRNA表达显著增加（P = 0.026）。得出的结论是，单独使用NPH胰岛素有助于伤口愈合，但没有必要将其与外源性NO气体联合使用。
