质粒DNA包裹于脂质体中。
Entrapment of plasmid DNA in liposomes.
作者信息
Dimitriadis G J
出版信息
Nucleic Acids Res. 1979 Jun 25;6(8):2697-705. doi: 10.1093/nar/6.8.2697.
Abstract
The entrapment of plasmid DNA (pMB9) and high molecular weight DNA into large unilamellar liposomes is described. The entrapment of DNA is specific and due to encapsulation of DNA into the aqueous compartment of liposomes. The entrapped Dna, resistant to deoxyribonuclease treatment, could be reisolated from liposomes intact and, as has been shown by transformation assay, it remains biologically active. The advantages of our method and possible applications are discussed.
摘要
本文描述了将质粒DNA（pMB9）和高分子量DNA包裹于大单层脂质体中的方法。DNA的包裹具有特异性，是由于DNA被包裹于脂质体的水相部分。包裹的DNA对脱氧核糖核酸酶处理具有抗性，可完整地从脂质体中重新分离出来，并且如转化试验所示，它仍保持生物活性。文中讨论了我们方法的优点及可能的应用。
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