畸胎癌衍生细胞系TerC表面抗原的免疫化学特性分析。
Immunochemical characterization of surface antigens of TerC, a teratocarcinoma-derived cell line.
作者信息
Larraga V, Edidin M
出版信息
Proc Natl Acad Sci U S A. 1979 Jun;76(6):2912-6. doi: 10.1073/pnas.76.6.2912.
Rabbit and mouse antisera prepared against teratocarcinoma cells precipitate both glycoproteins and glycolipids from detergent extracts of radiolabeled cells. Extracts of immunoprecipitates with chloroform/methanol, 2:1 (vol/vol) have been resolved on thin-layer gels into multiple peaks. There are more species seen in extracts of teratocarcinoma cells than in extracts of the crossreacting cultured cell line, cl 1d. The teratocarcinoma antigens may be extracted out of chloroform/methanol into buffered saline. Incubation in these secondary extracts converts unreactive cells (lymphocytes to cells reactive with antisera against teratocarcinoma. Furthermore, the coated cells absorb at least 80% of the activity of antisera against teratocarcinoma targets.
针对畸胎癌细胞制备的兔和小鼠抗血清可从放射性标记细胞的去污剂提取物中沉淀糖蛋白和糖脂。用氯仿/甲醇（2:1，体积/体积）对免疫沉淀物提取物进行薄层凝胶分离，得到多个峰。与交叉反应的培养细胞系cl 1d提取物相比，在畸胎癌细胞提取物中观察到更多种类。畸胎癌抗原可从氯仿/甲醇中提取到缓冲盐溶液中。在这些二级提取物中孵育可使无反应性细胞（淋巴细胞）转变为与抗畸胎癌抗血清有反应的细胞。此外，包被的细胞可吸收至少80%的抗畸胎癌靶标抗血清活性。
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