Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China.
University of Chinese Academy of Sciences, Beijing 100049, PR China.
Int J Syst Evol Microbiol. 2023 Nov;73(11). doi: 10.1099/ijsem.0.006113.
A floc-forming bacterial strain, designated HF-7, was isolated from the activated sludge of an industrial wastewater treatment plant in Hefei, PR China. Cells of this strain were Gram-stain-positive, catalase- and oxidase-negative, facultatively anaerobic, and rod-shaped. Growth occurred at 20-42 °C (optimum, 28 °C), at pH 5.5-10.5 (optimum, pH 7.5) and with 0-8.0 % (w/v) NaCl (optimum, 1 %). The major fatty acid was anteiso-C. The polar lipid profile contained phosphatidylglycerol, diphosphatidylglycerol and phosphatidylinositol. The DNA G+C content was 67 mol% from whole genomic sequence analysis. Based on the results of 16S rRNA gene sequence analysis, this strain should be assigned to the genus and is closely related to CAU 1319 (95.87 % similarity), IPBSL-7 (95.19 %) and Ben 106 (94.63 %) but separated from them by large distances in different phylogenetic trees. Based on whole genome analysis, the orthologous average nucleotide identity and DNA-DNA hybridization values against two of the closest relatives were 75.21-76.50 % and 14.2-24.4 %, respectively. The phylogenetic, genotypic, phenotypic and chemotaxonomic data demonstrated that strain HF-7 could be distinguished from its phylogenetically related species and represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is HF-7 (=KCTC 49959=CCTCC AB 2023019).
一株絮状形成细菌菌株，命名为 HF-7，从中国安徽合肥的一家工业废水处理厂的活性污泥中分离得到。该菌株的细胞革兰氏阳性，过氧化氢酶和氧化酶阴性，兼性厌氧，呈杆状。生长温度为 20-42℃（最适温度为 28℃），pH 值为 5.5-10.5（最适 pH 值为 7.5），盐度为 0-8.0%（w/v）（最适盐度为 1%）。主要脂肪酸为 anteiso-C。极性脂类图谱含有磷脂酰甘油、双磷脂酰甘油和磷脂酰肌醇。DNA G+C 含量为 67 mol%，来自全基因组序列分析。根据 16S rRNA 基因序列分析结果，该菌株应归属于 属，与 CAU 1319（95.87%相似性）、 IPBSL-7（95.19%）和 Ben 106（94.63%）最为接近，但在不同的系统发育树中与它们相距较远。基于全基因组分析，与两个最接近的亲缘关系菌株的直系同源平均核苷酸同一性和 DNA-DNA 杂交值分别为 75.21-76.50%和 14.2-24.4%。系统发育、基因型、表型和化学分类学数据表明，菌株 HF-7 可以与亲缘关系较近的种区分开来，代表了 属内的一个新种，因此建议使用 sp. nov. 作为新种的名称。模式菌株为 HF-7（=KCTC 49959=CCTCC AB 2023019）。
