School of Safety Science and Engineering, Henan Polytechnic University, Jiaozuo, 454003, China.
State Key Laboratory Cultivation Base for Gas Geology and Gas Control, Henan Polytechnic University, Jiaozuo, 454003, China.
Sci Rep. 2023 Nov 23;13(1):20537. doi: 10.1038/s41598-023-47787-1.
Bacterial sulfate reduction (BSR) is one of the key factors leading to the anomalous accumulation of hydrogen sulphide in coal mines. Environmental factors such as temperature and pH play a crucial role in the metabolism and degradation of coal by sulfate-reducing bacteria (SRB). In this study, coal samples were selected from Shengli Coal Mine, and SRB strains were isolated and purified from mine water using a dilution spread-plate anaerobic cultivation method. Based on single-factor experiments and response surface methodology (RSM), the impact of temperature, pH, oxidation-reduction potential (ORP), chemical oxygen demand to sulfate ratio (COD/SO) on the generation of hydrogen sulphide during brown coal BSR was analyzed. The results showed that the anaerobic degradation of coal by SRB was inhibited by either too high or too low a temperature to produce hydrogen sulfide, and the greatest production of hydrogen sulfide occurred at a temperature of about 30 °C; The greatest production of hydrogen sulfide occurred at an initial ambient pH of 7.5; COD/SO ratio of around 2.0 is most conducive to hydrogen sulphide generation; the lower ORP value is more favorable for hydrogen sulfide generation. The optimal conditions obtained by RSM were: temperature of 30.37 °C, pH of 7.64 and COD/SO of 1.96. Under these conditions, the hydrogen sulfide concentration was 56.79 mg/L, the pH value was 8.40, the ORP value was -274 mV, and the SO utilization rate was 58.04%. The RSM results showed that temperature, ambient pH and COD/SO had a significant effect on hydrogen sulfide production, and the degree of effect was: ambient pH > temperature > COD/SO.
细菌硫酸盐还原（BSR）是导致煤矿中硫化氢异常积累的关键因素之一。环境因素，如温度和 pH 值，在硫酸盐还原菌（SRB）对煤的代谢和降解中起着至关重要的作用。本研究从胜利煤矿选取煤样，采用稀释平板厌氧培养法从矿水中分离和纯化硫酸盐还原菌（SRB）菌株。基于单因素实验和响应面法（RSM），分析了温度、pH 值、氧化还原电位（ORP）、化学需氧量与硫酸盐比（COD/SO）对褐煤 BSR 过程中产生硫化氢的影响。结果表明，SRB 对煤的厌氧降解作用受到温度过高或过低的抑制，而产生硫化氢的最大量发生在温度约为 30°C；初始环境 pH 值为 7.5 时产生的硫化氢最大；COD/SO 比约为 2.0 最有利于产生硫化氢；较低的 ORP 值更有利于产生硫化氢。RSM 得到的最佳条件为：温度 30.37°C，pH 值 7.64，COD/SO 为 1.96。在此条件下，硫化氢浓度为 56.79mg/L，pH 值为 8.40，ORP 值为-274mV，SO 利用率为 58.04%。RSM 结果表明，温度、环境 pH 值和 COD/SO 对硫化氢生成有显著影响，影响程度为：环境 pH 值>温度>COD/SO。
