Wang Wei, Zhao Wenbin, Cao Bingbing, Liu Ronghua, Cheng Haojin, Tian Peng, Xiao Jiaguang, Xu Ziqing, Wang Xiaolei, Niu Wentao
Laboratory of Marine Biology and Ecology, Third Institute of Oceanography, Ministry of Natural Resources, 178 Daxue Road, Xiamen 361005, PR China.
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
Int J Syst Evol Microbiol. 2023 Feb;73(1). doi: 10.1099/ijsem.0.005676.
A Gram-straining-negative, facultatively anaerobic, motile by means of a polar flagellum and rod-shaped marine bacterium, designated S4M6, was isolated from surface seawater collected in Dongshan Bay (Fujian, PR China). Phylogenetic analysis based on 16S rRNA genes, phylogenomic analysis of single-copy gene families and whole genome data indicated that S4M6 represented a member of the genus . The closest phylogenetic relatives of S4M6 were CGMCC 1.8994 (97.8 % 16S rRNA gene sequence pairwise similarity), LMG 25438 (96.9 %), SZDIS-1 (96.2 %) and M22 (96.1 %). The growth of S4M6 occurred at 15-35 °C (optimum 28 °C), pH 4.0-9.0 (optimum 5.0-7.0) and in the presence of 2-5 % (w/v) NaCl (optimum 3 %). The predominant fatty acids (>10 %) are C, summed feature 3 (Cω7 and/or C ω6c) and summed feature 8 (Cω7 and/or C ω6). The DNA G+C content of the assembled genomic sequences was 43.4 % for S4M6. Average nucleotide identity (ANI) values between S4M6 and the reference species were lower than the threshold for species delineation (95-96 %); DNA-DNA hybridization further indicated that S4M6 had less than 70 % similarity to its relatives. On the basis of the polyphasic evidence, strain S4M6 is proposed to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S4M6 (= KCTC 92312= MCCC 1K06167).
从中国福建省东山湾采集的表层海水中分离出一株革兰氏染色阴性、兼性厌氧、通过极生鞭毛运动的杆状海洋细菌，命名为S4M6。基于16S rRNA基因的系统发育分析、单拷贝基因家族的系统基因组分析和全基因组数据表明，S4M6代表该属的一个成员。S4M6最接近的系统发育亲缘关系菌是CGMCC 1.8994（16S rRNA基因序列成对相似性为97.8%）、LMG 25438（96.9%）、SZDIS-1（96.2%）和M22（96.1%）。S4M6在15-35°C（最适温度28°C）、pH 4.0-9.0（最适pH 5.0-7.0）以及2-5%（w/v）NaCl（最适3%）存在的条件下生长。主要脂肪酸（>10%）为C、总和特征3（Cω7和/或Cω6c）和总和特征8（Cω7和/或Cω6）。S4M6组装基因组序列的DNA G+C含量为43.4%。S4M6与参考物种之间的平均核苷酸同一性（ANI）值低于物种界定阈值（95-96%）；DNA-DNA杂交进一步表明，S4M6与其亲缘关系菌的相似性低于70%。基于多相证据，菌株S4M6被提议代表该属的一个新物种，为此提议命名为sp. nov.。模式菌株为S4M6（=KCTC 92312 = MCCC 1K06167）。
