Karon A J, Bailey Emily S, Sobsey Mark D
Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Department of Public Health, College of Pharmacy & Health Sciences, Campbell University, Buies Creek, NC 27506, USA.
Pathogens. 2024 Jun 21;13(7):526. doi: 10.3390/pathogens13070526.
Current and emerging legislation in North Carolina and other regions calls for the enumeration of as a surrogate indicator for protozoan parasites in various types of waters. Past studies that have evaluated selective media for the detection of this bacterium have provided limited, conflicting, and inconclusive results. In this study, membrane filtration was used to enumerate as culturable spores or total culturable cells in 19 samples of untreated and 25 samples of partially treated wastewaters on 3 candidate media, Tryptose Sulfite Cycloserine Agar (TSC), CP ChromoSelect Agar (CPCS), and membrane Agar (m-CP) in parallel, and the results were compared. Presumptive isolates from each agar were further subjected to phenotypic confirmation tests for acid phosphatase production and stormy fermentation to further determine the performance of each agar. The CPCS agar was determined to have the highest enumerative capacity of total cells when compared to both TSC agar and m-CP agar (-value < 0.05), but there was no significant difference in its ability to detect spores when compared to TSC agar (-value > 0.05). The overall specificity of CPCS agar as determined by agreement of results from both confirmation tests was 0.81, while the specificity of TSC agar was only 0.28. Based on its performance, ease of preparation and use and consistency of colony characteristics, CPCS agar is recommended as the preferred medium for enumeration in wastewater.
北卡罗来纳州和其他地区现行及新出现的法规要求对 进行计数，将其作为各类水体中原生动物寄生虫的替代指标。过去评估用于检测这种细菌的选择性培养基的研究结果有限、相互矛盾且无定论。在本研究中，采用膜过滤法，在3种候选培养基（胰蛋白胨亚硫酸盐环丝氨酸琼脂（TSC）、CP色选琼脂（CPCS）和膜 琼脂（m-CP））上，并行计数19份未处理废水样品和25份部分处理废水样品中的可培养孢子或总可培养细胞，并对结果进行比较。对每种琼脂上的推定分离株进一步进行酸性磷酸酶产生和剧烈发酵的表型确认试验，以进一步确定每种琼脂的性能。与TSC琼脂和m-CP琼脂相比，CPCS琼脂对总 细胞的计数能力最高（-值<0.05），但与TSC琼脂相比，其检测孢子的能力无显著差异（-值>0.05）。通过两项确认试验结果的一致性确定，CPCS琼脂的总体特异性为0.81，而TSC琼脂的特异性仅为0.28。基于其性能、制备和使用的简便性以及菌落特征的一致性，建议将CPCS琼脂作为废水中 计数的首选培养基。
