Sartory D P, Field M, Curbishley S M, Pritchard A M
Severn Trent Water, Quality & Environmental Services, Shrewsbury, UK.
Lett Appl Microbiol. 1998 Dec;27(6):323-7. doi: 10.1046/j.1472-765x.1998.00454.x.
Two media (mCP medium and Tryptose Sulphite Cycloserine (TSC) agar) were evaluated for recovery of Clostridium perfringens in environmental and part-treated drinking water. For laboratory strains of Clostridium, mCP was more selective and specific for Cl. perfringens than TSC, but was markedly less efficient for the enumeration of both vegetative cells and spores. For samples of river water and part-treated drinking water, TSC recovered significantly greater numbers of Cl. perfringens than mCP. In contrast to previous reports, there was a significant number of false presumptive positive and negative isolates on mCP. TSC is a more suitable medium for the routine monitoring of water supplies for the presence of Cl. perfringens.
评估了两种培养基（mCP培养基和胰蛋白胨亚硫酸盐环丝氨酸（TSC）琼脂）用于从环境和部分处理过的饮用水中回收产气荚膜梭菌的效果。对于产气荚膜梭菌的实验室菌株，mCP对产气荚膜梭菌的选择性和特异性比TSC更高，但在计数营养细胞和芽孢方面的效率明显较低。对于河水和部分处理过的饮用水样本，TSC回收的产气荚膜梭菌数量明显多于mCP。与之前的报告相反，mCP上有大量假阳性和假阴性的推定分离株。TSC是用于常规监测供水系统中是否存在产气荚膜梭菌的更合适培养基。
