Jovanovic Nemanja M, Glavinic Uros, Ristanic Marko, Vejnovic Branislav, Ilic Tamara, Stevanovic Jevrosima, Stanimirovic Zoran
Department of Parasitology, Faculty of Veterinary Medicine, University of Belgrade, Bul. Oslobodjenja 18, 11000 Belgrade, Serbia.
Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Bul. Oslobodjenja 18, 11000 Belgrade, Serbia.
Animals (Basel). 2023 Nov 16;13(22):3543. doi: 10.3390/ani13223543.
One of the most important approaches in the prevention and treatment of nosemosis is the use of herbal preparations as food supplements for bees. Therefore, the aim of this study was to investigate the effects of a plant-based supplement branded as "B+" on honeybees in a laboratory experiment. Four experimental groups were established: treated group (T), -infected and treated group (IT), -infected group (I) and non-infected group (NI). Survival, spore load and oxidative stress parameters together with expression levels of antioxidant enzyme genes and vitellogenin gene were monitored. The mortality in the T, IT and NI groups was significantly ( < 0.001) lower than in than in the I group. Within -infected groups, the IT group had a significantly lower ( < 0.001) number of spores than the I group. In addition, expression levels of genes for antioxidant enzymes were lower ( < 0.001) in the IT group compared to the I group. The concentration of malondialdehyde and the activities of antioxidant enzymes (superoxide dismutase, catalase and glutathione S-transferase) were significantly lower ( < 0.001) in the IT group compared to the I group. No negative effects of the tested supplement were observed. All these findings indicate that the tested supplement exerted beneficial effects manifested in better bee survival, reduced spore number and reduced oxidative stress of bees (lower expression of genes for antioxidant enzymes and oxidative stress parameters).
预防和治疗蜜蜂微孢子虫病最重要的方法之一是使用草药制剂作为蜜蜂的食物补充剂。因此，本研究的目的是在实验室实验中研究一种名为“B+”的植物性补充剂对蜜蜂的影响。设立了四个实验组：处理组（T）、感染并处理组（IT）、感染组（I）和未感染组（NI）。监测了存活率、孢子载量和氧化应激参数以及抗氧化酶基因和卵黄原蛋白基因的表达水平。T组、IT组和NI组的死亡率显著低于I组（<0.001）。在感染组中，IT组的孢子数量显著低于I组（<0.001）。此外，与I组相比，IT组抗氧化酶基因的表达水平更低（<0.001）。与I组相比，IT组丙二醛浓度和抗氧化酶（超氧化物歧化酶、过氧化氢酶和谷胱甘肽S-转移酶）活性显著降低（<0.001）。未观察到受试补充剂的负面影响。所有这些发现表明，受试补充剂发挥了有益作用，表现为蜜蜂存活率提高、孢子数量减少以及蜜蜂氧化应激减轻（抗氧化酶基因表达和氧化应激参数降低）。
