Glavinic Uros, Rajkovic Milan, Vunduk Jovana, Vejnovic Branislav, Stevanovic Jevrosima, Milenkovic Ivanka, Stanimirovic Zoran
Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobodjenja 18, 11000 Belgrade, Serbia.
Faculty of Agriculture, Institute for Food Technology and Biochemistry, University of Belgrade, Nemanjina 6, 11080 Belgrade, Serbia.
Insects. 2021 Oct 7;12(10):915. doi: 10.3390/insects12100915.
water crude extract was tested on honey bees for the first time. The first part of the cage experiment was set for selecting one concentration of the extract. Concentration of 200 µg/g was further tested in the second part of the experiment where bee survival and food consumption were monitored together with infection level and expression of five genes (abaecin, hymenoptaecin, defensin, apidaecin, and vitellogenin) that were evaluated in bees sampled on days 7 and 15. Survival rate of -infected bees was significantly greater in groups fed with -enriched syrup compared to those fed with a pure sucrose syrup. Besides, the anti- effect of extract was greatest when applied from the third day which coincides with the time of infection with Daily food consumption did not differ between the groups indicating good acceptability and palatability of the extract. extract showed a stimulative effect on four out of five monitored genes. Both anti- and nutrigenomic effects of extract were observed when supplementation started at the moment of infection or preventively (before or simultaneously with the infection).
首次对蜜蜂进行了水粗提物测试。笼养实验的第一部分旨在选择提取物的一种浓度。在实验的第二部分中，对200 µg/g的浓度进行了进一步测试，在该部分中，监测了蜜蜂的存活率和食物消耗情况，同时还监测了在第7天和第15天采集的蜜蜂的感染水平以及五个基因（abaecin、hymenoptaecin、defensin、apidaecin和卵黄蛋白原）的表达情况。与喂食纯蔗糖糖浆的蜜蜂相比，喂食富含该提取物糖浆的感染蜜蜂组的存活率显著更高。此外，当从第三天开始应用该提取物时，其抗（感染）效果最佳，这与感染时间相吻合。各组之间的每日食物消耗量没有差异，表明该提取物具有良好的可接受性和适口性。该提取物对五个监测基因中的四个显示出刺激作用。当在感染时开始补充或预防性地（在感染之前或同时）补充该提取物时，观察到了其抗（感染）和营养基因组效应。
