Merlo Barbara, Iacono Eleonora
Department of Veterinary Medical Sciences, University of Bologna, 40064 Bologna, Italy.
Interdepartmental Centre for Industrial Research in Health Sciences and Technologies, University of Bologna, 40126 Bologna, Italy.
Animals (Basel). 2023 Nov 19;13(22):3571. doi: 10.3390/ani13223571.
A dog is a valuable animal model and concomitantly a pet for which advanced therapies are increasingly in demand. The characteristics of mesenchymal stem/stromal cells (MSCs) have made cell therapy more clinically attractive. During the last decade, research on the MSC therapeutic effectiveness has demonstrated that tissue regeneration is primarily mediated by paracrine factors, which are included under the name of secretome. Secretome is a mixture of soluble factors and a variety of extracellular vesicles. The use of secretome for therapeutic purposes could have some advantages compared to cell-based therapies, such as lower immunogenicity and easy manufacturing, manipulation, and storage. The conditioned medium and extracellular vesicles derived from MSCs have the potential to be employed as new treatments in veterinary medicine. This review provides an update on the state-of-the-art characterization and applications of canine adipose tissue-derived MSC secretome.
狗是一种有价值的动物模型，同时也是一种宠物，对其先进疗法的需求日益增加。间充质干/基质细胞（MSCs）的特性使细胞疗法在临床上更具吸引力。在过去十年中，对MSCs治疗效果的研究表明，组织再生主要由旁分泌因子介导，这些旁分泌因子统称为分泌组。分泌组是可溶性因子和多种细胞外囊泡的混合物。与基于细胞的疗法相比，将分泌组用于治疗目的可能具有一些优势，例如免疫原性较低以及易于制造、操作和储存。源自MSCs的条件培养基和细胞外囊泡有潜力用作兽医学的新疗法。本综述提供了犬脂肪组织来源的MSCs分泌组的最新表征和应用情况。
