Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, 13566-590, Brazil.
Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, 13566-590, Brazil; Instituto Nacional de Ciência e Tecnologia de Bioanalítica-INCTBio, Campinas, SP, 13083-970, Brazil.
Biosens Bioelectron. 2024 Feb 15;246:115846. doi: 10.1016/j.bios.2023.115846. Epub 2023 Nov 20.
The use of microfluidic paper-based analytical devices (μPADs) for aiding medical diagnosis is a growing trend in the literature mainly due to their low cost, easy use, simple manufacturing, and great potential for application in low-resource settings. Many important biomarkers (proteins, ions, lipids, hormones, DNA, RNA, drugs, whole cells, and more) and biofluids are available for precise detection and diagnosis. We have reviewed the advances μPADs in medical diagnostics have achieved in the last few years, focusing on the most common human biofluids (whole blood/plasma, sweat, urine, tears, and saliva). The challenges of detecting specific biomarkers in each sample are discussed, along with innovative techniques that overcome such limitations. Finally, the difficulties of commercializing μPADs are considered, and future trends are presented, including wearable devices and integrating multiple steps in a single platform.
微流控纸基分析器件（μPADs）在医学诊断中的应用是文献中的一个新兴趋势，主要是因为它们具有低成本、易用、简单制造以及在资源匮乏环境中应用的巨大潜力。许多重要的生物标志物（蛋白质、离子、脂质、激素、DNA、RNA、药物、全细胞等）和生物流体可用于精确检测和诊断。我们回顾了过去几年μPADs 在医学诊断方面取得的进展，重点关注最常见的人体生物流体（全血/血浆、汗液、尿液、眼泪和唾液）。讨论了在每种样本中检测特定生物标志物所面临的挑战，以及克服这些限制的创新技术。最后，考虑了 μPADs 商业化所面临的困难，并提出了未来的趋势，包括可穿戴设备和在单个平台上集成多个步骤。
