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人结肠mRNA序列的克隆：正常和肿瘤组织中特定mRNA的初步特征分析

Cloning of mRNA sequences from the human colon: preliminary characterisation of defined mRNAs in normal and neoplastic tissues.

作者信息

Bartsch R A, Joannou C, Talbot I C, Bailey D S

出版信息

Br J Cancer. 1986 Nov;54(5):791-8. doi: 10.1038/bjc.1986.242.

DOI:10.1038/bjc.1986.242
PMID:3801274
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2001548/
Abstract

RNA has been extracted from the normal human colon, converted into cDNA and cloned in the bacterial plasmid pBR322. About 4,000 sequences from this library were screened with probes derived from normal mucosa, familial polyposis mucosa, colonic adenocarcinomas and the colon tumour cell line HT29. Some mucosal sequences showed greatly reduced levels of transcription in neoplastic conditions, while a few showed elevated transcription. These have been further characterised by Northern and RNA dot-blot analysis.

摘要

已从正常人结肠中提取RNA，将其转化为cDNA并克隆到细菌质粒pBR322中。用来自正常黏膜、家族性息肉病黏膜、结肠腺癌和结肠肿瘤细胞系HT29的探针筛选了该文库中的约4000个序列。一些黏膜序列在肿瘤状态下转录水平大幅降低，而少数序列转录水平升高。已通过Northern印迹和RNA斑点印迹分析对这些序列进行了进一步表征。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6158/2001548/25c05b8627ff/brjcancer00522-0077-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6158/2001548/c5f1e7259dc5/brjcancer00522-0073-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6158/2001548/4d99635cc42a/brjcancer00522-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6158/2001548/fb42fc45fdb7/brjcancer00522-0076-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6158/2001548/25c05b8627ff/brjcancer00522-0077-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6158/2001548/c5f1e7259dc5/brjcancer00522-0073-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6158/2001548/4d99635cc42a/brjcancer00522-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6158/2001548/fb42fc45fdb7/brjcancer00522-0076-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6158/2001548/25c05b8627ff/brjcancer00522-0077-a.jpg

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引用本文的文献

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2
Isolation and preliminary characterisation of cDNA clones representing mRNAs associated with tumour progression and metastasis in colorectal cancer.代表与结直肠癌肿瘤进展和转移相关的信使核糖核酸的互补脱氧核糖核酸克隆的分离及初步特性分析
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Ribosomal protein genes are overexpressed in colorectal cancer: isolation of a cDNA clone encoding the human S3 ribosomal protein.

本文引用的文献

1
Pathology of colorectal adenomas: a colonoscopic survey.结直肠腺瘤的病理学：一项结肠镜检查调查。
J Clin Pathol. 1982 Aug;35(8):830-41. doi: 10.1136/jcp.35.8.830.
2
Cloning and screening of sequences expressed in a mouse colon tumor.小鼠结肠肿瘤中表达序列的克隆与筛选
Cancer Res. 1982 Mar;42(3):1088-93.
3
Hormone-dependent terminal differentiation in vitro of chicken erythroleukemia cells transformed by ts mutants of avian erythroblastosis virus.禽成红细胞增多症病毒ts突变体转化的鸡红细胞白血病细胞在体外的激素依赖性终末分化
核糖体蛋白基因在结直肠癌中过表达：编码人S3核糖体蛋白的cDNA克隆的分离
Mol Cell Biol. 1991 Aug;11(8):3842-9. doi: 10.1128/mcb.11.8.3842-3849.1991.
Cell. 1982 Apr;28(4):907-19. doi: 10.1016/0092-8674(82)90070-8.
4
Number and evolutionary conservation of alpha- and beta-tubulin and cytoplasmic beta- and gamma-actin genes using specific cloned cDNA probes.使用特异性克隆的cDNA探针检测α-和β-微管蛋白以及细胞质β-和γ-肌动蛋白基因的数量和进化保守性。
Cell. 1980 May;20(1):95-105. doi: 10.1016/0092-8674(80)90238-x.
5
Histochemistry of epithelial metaplasia and dysplasia in human stomach and colorectum.人胃和结肠上皮化生及发育异常的组织化学
Scand J Gastroenterol Suppl. 1984;104:109-30.
6
Laminin and fibronectin in rectal adenocarcinoma: relationship to tumour grade, stage and metastasis.层粘连蛋白和纤连蛋白在直肠腺癌中的作用：与肿瘤分级、分期及转移的关系
Br J Cancer. 1984 Jul;50(1):51-61. doi: 10.1038/bjc.1984.139.
7
Adenomas: preneoplastic events, growth and development in man and experimental systems.腺瘤：人类及实验系统中的肿瘤前病变、生长与发展
Pathol Annu. 1983;18 Pt 1:205-19.
8
Studies on transformation of Escherichia coli with plasmids.大肠杆菌质粒转化的研究。
J Mol Biol. 1983 Jun 5;166(4):557-80. doi: 10.1016/s0022-2836(83)80284-8.
9
Close similarity of epidermal growth factor receptor and v-erb-B oncogene protein sequences.表皮生长因子受体与v-erb-B癌基因蛋白序列的高度相似性。
Nature. 1984;307(5951):521-7. doi: 10.1038/307521a0.
10
Activation of mouse genes in transformed cells.转化细胞中小鼠基因的激活。
Cell. 1983 Sep;34(2):557-67. doi: 10.1016/0092-8674(83)90388-4.