Haghighian Niloofar, Kataky Ritu
Department of Chemistry, University of Durham Lower Mountjoy Durham DH1 3LE UK
Sens Diagn. 2023 Jun 16;2(5):1228-1235. doi: 10.1039/d3sd00074e. eCollection 2023 Sep 14.
Rapid and precise identification of infectious microorganisms is important across a range of applications where microbial contamination can cause serious issues ranging from microbial resistance to corrosion. In this paper a screen-printed, polymeric β-cyclodextrin (β-CD) modified electrode, affording nanocavities for inclusion of the analytes, is shown as a disposable sensor capable of identifying bacteria by their metabolites. Three bacterial species were tested: two from the genus, () and (), and (), a member of the family, . On biofilm formation each species gave distinct, reproducible, redox fingerprints with a detection limit of 4 × 10 M. Square wave adsorptive stripping voltammetry (SWAdSV) was used for detection. Scanning electron microscopy (SEM) and cyclic voltammetry (CV) techniques were used to characterize the morphology and electrical conductivity of the modified electrode. In comparison to the bare screen-printed electrode, the modified electrode showed a considerably higher performance and offered an excellent sensitivity along with a relatively fast analysis time.
在一系列微生物污染会引发严重问题（从微生物抗药性到腐蚀）的应用中，快速准确地识别感染性微生物至关重要。本文展示了一种丝网印刷的、聚合物β-环糊精（β-CD）修饰电极，该电极提供用于容纳分析物的纳米腔，作为一种能够通过细菌代谢物识别细菌的一次性传感器。测试了三种细菌：两种来自属（）和（），以及（），科的一个成员。在生物膜形成时，每个物种都给出了独特的、可重复的氧化还原指纹图谱，检测限为4×10 M。采用方波吸附溶出伏安法（SWAdSV）进行检测。利用扫描电子显微镜（SEM）和循环伏安法（CV）技术对修饰电极的形态和电导率进行表征。与裸丝网印刷电极相比，修饰电极表现出显著更高的性能，具有出色的灵敏度以及相对较快的分析时间。
