N-酰基鞘磷脂囊泡的X射线散射。双层厚度的测定。
X-ray scattering of vesicles of N-acyl sphingomyelins. Determination of bilayer thickness.
作者信息
Maulik P R, Atkinson D, Shipley G G
出版信息
Biophys J. 1986 Dec;50(6):1071-7. doi: 10.1016/S0006-3495(86)83551-2.
Abstract
A series of N-acyl sphingomyelins (C16:0, C18:0, C20:0, C22:0, and C24:0) have been synthesized and single bilayer vesicles formed by sonication and ultracentrifugation. X-ray scattering data have been collected from the sphingomyelin vesicles at 50 degrees C in the melted-chain state. The x-ray scattering data have been transformed to the corresponding Patterson functions and Fourier electron density profiles; analysis of these functions has provided the intrabilayer phosphate-phosphate separation dp-p, a measure of the lipid bilayer thickness. The bilayer thickness increases linearly with increasing chain length (increment 1.3-1.4 A) and the intercept, 14.3-15.0 A, suggests a contribution of 7.0-7.5 A for each phosphorylcholine group to the bilayer thickness. The electron-density profiles have features suggestive of chain interdigitation when the length of the N-acyl chain (C20:0, C22:0, and C24:0) exceeds significantly the length of the invariant sphingosine chain.
摘要
已合成了一系列N-酰基鞘磷脂（C16:0、C18:0、C20:0、C22:0和C24:0），并通过超声处理和超速离心形成了单层囊泡。在50摄氏度的熔链状态下，从鞘磷脂囊泡收集了X射线散射数据。已将X射线散射数据转换为相应的帕特森函数和傅里叶电子密度分布图；对这些函数的分析提供了双层内磷酸根-磷酸根间距dp-p，这是脂质双层厚度的一种度量。双层厚度随链长增加呈线性增加（增量为1.3 - 1.4 Å），截距为14.3 - 15.0 Å，表明每个磷酸胆碱基团对双层厚度的贡献为7.0 - 7.5 Å。当N-酰基链（C20:0、C22:0和C24:0）的长度显著超过不变的鞘氨醇链的长度时，电子密度分布图具有链相互交错的特征。
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