State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, International School of Materials Science and Engineering, Wuhan University of Technology, Wuhan 430070, P. R. China.
School of Medicine, Wuhan University of Science and Technology, Wuhan 430081, P. R. China.
Sci Adv. 2023 Dec;9(48):eadk7251. doi: 10.1126/sciadv.adk7251. Epub 2023 Nov 29.
Biocompatible swarming magnetic nanorobots that work in blood vessels for safe and efficient targeted thrombolytic therapy in vivo are demonstrated. This is achieved by using magnetic beads elaborately grafted with heparinoid-polymer brushes (HPBs) upon the application of an alternating magnetic field (). Because of the dense surface charges bestowed by HPBs, the swarming nanorobots demonstrate reversible agglomeration-free reconfigurations, low hemolysis, anti-bioadhesion, and self-anticoagulation in high-ionic-strength blood environments. They are confirmed in vitro and in vivo to perform synergistic thrombolysis efficiently by "motile-targeting" drug delivery and mechanical destruction. Moreover, upon the completion of thrombolysis and removal of (), the nanorobots disassemble into dispersed particles in blood, allowing them to safely participate in circulation and be phagocytized by immune cells without apparent organ damage or inflammatory lesion. This work provides a rational multifaceted HPB biointerfacing design strategy for biomedical nanorobots and a general motile platform to deliver drugs for targeted therapies.
展示了在血管中工作的生物相容的群体运动磁性纳米机器人，用于体内安全有效的靶向溶栓治疗。这是通过在施加交变磁场 () 时，在磁珠上精心接枝肝素类聚合物刷 (HPB) 来实现的。由于 HPB 赋予的密集表面电荷，群体纳米机器人表现出可逆的无聚集再配置、低溶血、抗生物黏附性和自抗凝性，在高离子强度的血液环境中。它们在体外和体内被证实通过“运动靶向”药物递送和机械破坏来有效地进行协同溶栓。此外，在溶栓完成和 () 去除后，纳米机器人在血液中分解为分散的颗粒，从而能够安全地参与循环并被免疫细胞吞噬，而不会造成明显的器官损伤或炎症损伤。这项工作为生物医学纳米机器人提供了一种合理的多方面的 HPB 生物界面设计策略，以及一种用于靶向治疗的通用运动平台来输送药物。
