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RNA 结合蛋白有助于植物细胞外囊泡中 small RNA 的加载。

RNA-binding proteins contribute to small RNA loading in plant extracellular vesicles.

机构信息

Department of Microbiology and Plant Pathology, Center for Plant Cell Biology, Institute for Integrative Genome Biology, University of California, Riverside, CA, USA.

State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan, China.

出版信息

Nat Plants. 2021 Mar;7(3):342-352. doi: 10.1038/s41477-021-00863-8. Epub 2021 Feb 25.

DOI:10.1038/s41477-021-00863-8
PMID:33633358
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7979528/
Abstract

Plants use extracellular vesicles (EVs) to transport small RNAs (sRNAs) into their fungal pathogens and silence fungal virulence-related genes through a phenomenon called 'cross-kingdom RNAi'. It remains unknown, however, how sRNAs are selectively loaded into EVs. Here, we identified several RNA-binding proteins in Arabidopsis, including Argonaute 1 (AGO1), RNA helicases (RHs) and annexins (ANNs), which are secreted by exosome-like EVs. AGO1, RH11 and RH37 selectively bind to EV-enriched sRNAs but not to non-EV-associated sRNAs, suggesting that they contribute to the selective loading of sRNAs into EVs. Conversely, ANN1 and ANN2 bind to sRNAs non-specifically. The ago1, rh11 rh37 and ann1 ann2 mutants showed reduced secretion of sRNAs in EVs, demonstrating that these RNA-binding proteins play an important role in sRNA loading and/or stabilization in EVs. Furthermore, rh11 rh37 and ann1 ann2 showed increased susceptibility to Botrytis cinerea, suggesting that RH11, RH37, ANN1 and ANN2 positively regulate plant immunity against B. cinerea.

摘要

植物利用细胞外囊泡（EVs）将小 RNA（sRNAs）运输到真菌病原体中，并通过一种称为“跨物种 RNAi”的现象沉默真菌致病相关基因。然而，目前尚不清楚 sRNAs 是如何被选择性地装载到 EVs 中的。在这里，我们鉴定了拟南芥中的几种 RNA 结合蛋白，包括 Argonaute 1（AGO1）、RNA 解旋酶（RHs）和膜联蛋白（ANNs），它们通过类外体 EV 分泌。AGO1、RH11 和 RH37 选择性地结合到 EV 富集的 sRNAs 上，但不结合非 EV 相关的 sRNAs，这表明它们有助于 sRNAs 选择性地装载到 EVs 中。相反，ANN1 和 ANN2 非特异性地结合 sRNAs。ago1、rh11 rh37 和 ann1 ann2 突变体在 EV 中 sRNAs 的分泌减少，表明这些 RNA 结合蛋白在 sRNA 的装载和/或稳定 EVs 中发挥重要作用。此外，rh11 rh37 和 ann1 ann2 对灰葡萄孢菌的敏感性增加，表明 RH11、RH37、ANN1 和 ANN2 正向调节植物对灰葡萄孢菌的免疫。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7349/7979528/ba8d36107830/nihms-1667199-f0006.jpg
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