Department of Microbiology and Immunology, University of Arkansas for Medical Sciences , Little Rock, Arkansas, USA.
Department of Biostatistics, University of Arkansas for Medical Sciences , Little Rock, Arkansas, USA.
Microbiol Spectr. 2024 Jan 11;12(1):e0314723. doi: 10.1128/spectrum.03147-23. Epub 2023 Dec 7.
Because biofilm formation is such a problematic feature of infections, much effort has been put into identifying biofilm inhibitors. However, the results observed with these compounds are often reported in isolation, and the methods used to assess biofilm formation vary between labs, making it impossible to assess relative efficacy and prioritize among these putative inhibitors for further study. The studies we report address this issue by directly comparing putative biofilm inhibitors using a consistent assay. This assay was previously shown to maximize biofilm formation, and the results observed with this assay have been proven to be relevant . Of the 19 compounds compared using this method, many had no impact on biofilm formation under these conditions. Indeed, only one proved effective at limiting biofilm formation without also inhibiting growth.
由于生物膜形成是感染的一个有问题的特征，因此人们投入了大量精力来识别生物膜抑制剂。然而，这些化合物的观察结果通常是孤立报道的，并且用于评估生物膜形成的方法在实验室之间也有所不同，因此无法评估这些假定抑制剂的相对效果，并对它们进行优先排序，以进一步研究。我们报告的研究通过使用一致的测定方法直接比较假定的生物膜抑制剂来解决这个问题。该测定法先前已被证明可以最大程度地促进生物膜形成，并且用该测定法观察到的结果已被证明是相关的。在所比较的 19 种化合物中，许多在这些条件下对生物膜形成没有影响。实际上，只有一种化合物在不抑制生长的情况下有效限制生物膜形成。
