Matossian-Rogers A
Clin Exp Immunol. 1979 Apr;36(1):38-45.
The inhibition of the macrophage spreading test, claimed to be an in vitro correlate of delayed hypersensitivity, was examined in guinea-pigs immunized with L. enriettii and L. tropica soluble antigens. Cells from peritoneal washings of the guinea-pigs were tested in presence of the homologous and heterologous antigens and also without antigen. Inhibition of macrophage spreading compared to control preparations was noted only in the presence of the homologous antigen when the skin test response of the donor animal was relatively small. The degree of inhibition decreased as the skin test volume increased and when skin test volumes were large there was actual stimulation of macrophage spreading, rather than inhibition. The addition of heterologous antigen to the peritoneal cell preparation always resulted in the augmentation of macrophage spreading above control levels. The possible mechanisms of this in vitro technique and its use as a taxonomic or diagnostic tool are discussed.
在豚鼠中，用恩氏利什曼原虫和热带利什曼原虫可溶性抗原免疫后，检测了巨噬细胞铺展试验的抑制情况，该试验据称是迟发型超敏反应的体外相关指标。从豚鼠腹腔冲洗液中获取的细胞，在同源和异源抗原存在以及无抗原的情况下进行测试。仅当供体动物的皮肤试验反应相对较小时，在同源抗原存在的情况下才观察到与对照制剂相比巨噬细胞铺展受到抑制。随着皮肤试验体积的增加，抑制程度降低，当皮肤试验体积较大时，实际上是巨噬细胞铺展受到刺激，而非抑制。向腹腔细胞制剂中添加异源抗原总是导致巨噬细胞铺展高于对照水平。讨论了这种体外技术的可能机制及其作为分类或诊断工具的用途。
