Belehu A, Poulter L W, Turk J L
Clin Exp Immunol. 1976 Apr;24(1):125-32.
Pretreatment of guinea-pigs with cyclophosphamide (Cy) (300 mg/kg) 3 days before cutaneous infection with Leishmania enriettii caused an increased intensity of the lesion at the site of infection and an increase in the incidence of widespread metastases. Decreased levels of circulating antibody were found from the first to fourth week after infection. Decreased delayed type hypersensitivity could only be detected beyond 4 weeks. Peritoneal macrophages obtained form guinea-pigs 3 days after Cy pretreatment showed increase rather than decreased ability to phagocytose L. enriettii. Phagocytosis of L. enriettii by peritoneal macrophages obtained from guinea-pigs pretreated with Cy 24 days previously was normal. It is suggested that more attention should be taken of antibody levels during the early phase of infection and that control of infection could be due to a synergism between antibody and cell-mediated immunity. Upset in the balance by suppression of either function might lead to the development of widespread metastatic lesions.
在用恩氏利什曼原虫进行皮肤感染前3天，用环磷酰胺（Cy）（300毫克/千克）对豚鼠进行预处理，导致感染部位病变强度增加，广泛转移的发生率上升。在感染后的第一周至第四周，循环抗体水平下降。迟发型超敏反应降低仅在4周后才能检测到。环磷酰胺预处理3天后从豚鼠获得的腹腔巨噬细胞吞噬恩氏利什曼原虫的能力增强而非降低。24天前用环磷酰胺预处理的豚鼠获得的腹腔巨噬细胞对恩氏利什曼原虫的吞噬作用正常。建议在感染早期应更多关注抗体水平，并且感染的控制可能归因于抗体与细胞介导免疫之间的协同作用。抑制任何一种功能导致的平衡失调可能会导致广泛转移性病变的发展。
