Bretan P N, Vigneron D B, Hricak H, McClure R D, Yen T S, Moseley M, Tanagho E A, James T L
Radiology. 1987 Mar;162(3):867-71. doi: 10.1148/radiology.162.3.3809506.
To evaluate the reliability of phosphorus-31 magnetic resonance (MR) spectroscopy in the assessment of acute testicular ischemia, vascular integrity, and spermatogenesis, the authors studied in vivo canine and primate testicles grouped as follows: group 1 testes (n = 8), in situ canine controls; group 2 (n = 11), canine testes subjected to warm ischemia; group 3, canine (n = 4) and primate (n = 4) testicles from hormone-treated animals. Group 1 control testicles showed high monophosphoester (MP) levels; low levels of inorganic phosphate (Pi), phosphodiester (PD), and phosphocreatine; and high levels of adenosine triphosphate (ATP). Group 2 testes revealed a time-dependent decay of MP/Pi ratios (from 2.1 to 0.70). Regeneration of ATP was noted in the acute reperfusion period. After 6 weeks of pituitary gonadotropin suppression, group 3 testes showed a significant decrease (P less than .05) in MP/PD ratios from a control level of 2.6 +/- 0.3 and a decrease in the MP/beta-ATP ratio from 2.4 +/- 0.1 to 1.8 +/- 0.3. P-31 MR spectroscopy appears to be a potential method for noninvasively assessing testicular ischemic injury and the metabolic integrity of spermatogenesis.
为评估磷-31磁共振（MR）波谱在急性睾丸缺血、血管完整性及生精功能评估中的可靠性，作者对犬和灵长类动物的睾丸进行了体内研究，分组如下：第1组睾丸（n = 8），犬原位对照；第2组（n = 11），经历热缺血的犬睾丸；第3组，来自激素处理动物的犬（n = 4）和灵长类（n = 4）睾丸。第1组对照睾丸显示单磷酸酯（MP）水平高；无机磷酸盐（Pi）、磷酸二酯（PD）和磷酸肌酸水平低；三磷酸腺苷（ATP）水平高。第2组睾丸显示MP/Pi比值呈时间依赖性下降（从2.1降至0.70）。在急性再灌注期观察到ATP的再生。垂体促性腺激素抑制6周后，第3组睾丸的MP/PD比值从对照水平的2.6±0.3显著下降（P<0.05），MP/β-ATP比值从2.4±0.1降至1.8±0.3。磷-31 MR波谱似乎是一种非侵入性评估睾丸缺血损伤和生精代谢完整性的潜在方法。
