1Faculdade de Farmácia, Universidade Federal da Bahia, Salvador, Brazil.
2Kennesaw State University, Kennesaw, Georgia.
Am J Trop Med Hyg. 2021 Apr 19;104(6):2038-2041. doi: 10.4269/ajtmh.20-1539.
Strongyloides stercoralis is a soil-transmitted nematode that can cause life-threatening conditions in immunocompromised persons. In the United States, strongyloidiasis should be considered mainly in immigrants, refugees, or travelers. The confirmatory laboratory diagnosis is usually performed by detecting larvae from the stool, duodenal material, and sputum. In persons who are immunocompromised with severe strongyloidiasis, adult worms and eggs can be detected from duodenal material. For serological diagnosis, most assays use crude antigens to detect anti-S. stercoralis IgG. Recently, recombinant proteins such as rSs-NIE-1 and rSs-IR have been used to detect IgG antibodies. We used rSs-NIE-1 and rSs-IR recombinant antigens to develop a biplex Western blot assay to detect the IgG4 antibody in individuals with strongyloidiasis. The sensitivities of rSs-NIE-1 and rSs-IR were 97.4% and 90.8%, respectively, whereas the specificities were 97.6% and 98%, respectively. In conclusion, the biplex rSs-NIE-1 and rSs-IR immunoblot performs well in detecting IgG4 antibody in S. stercoralis-infected persons.
粪类圆线虫是一种土壤传播的线虫，可在免疫功能低下者中引起危及生命的情况。在美国，粪类圆线虫病主要应考虑发生在移民、难民或旅行者中。确认性实验室诊断通常通过检测粪便、十二指肠材料和痰液中的幼虫来进行。在严重粪类圆线虫感染的免疫功能低下者中，可从十二指肠材料中检测到成虫和虫卵。对于血清学诊断，大多数检测方法使用粗抗原来检测抗粪类圆线虫 IgG。最近，重组蛋白如 rSs-NIE-1 和 rSs-IR 已被用于检测 IgG 抗体。我们使用 rSs-NIE-1 和 rSs-IR 重组抗原开发了双相 Western blot 检测方法，以检测粪类圆线虫感染者的 IgG4 抗体。rSs-NIE-1 和 rSs-IR 的灵敏度分别为 97.4%和 90.8%，特异性分别为 97.6%和 98%。总之，双相 rSs-NIE-1 和 rSs-IR 免疫印迹在检测粪类圆线虫感染者的 IgG4 抗体方面表现良好。
