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研究单核细胞在淋巴细胞刺激中作用的显微技术

Microtechnique for studies on the role of monocytes in the stimulation of lymphocytes.

作者信息

Tärnvik A

出版信息

Infect Immun. 1979 Jun;24(3):589-92. doi: 10.1128/iai.24.3.589-592.1979.

DOI:10.1128/iai.24.3.589-592.1979
PMID:381194
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC414346/
Abstract

A microtiter technique was used to measure lymphocyte stimulation by antigen-pulsed monocytes. Monocytes from human blood were isolated on polyester film disks. The disks were exposed to antigen and incubated together with lymphocytes in the wells of a microtiter plate. The method was technically simple and required no expensive materials.

摘要

采用微量滴定技术来测定经抗原刺激的单核细胞对淋巴细胞的刺激作用。用人血中的单核细胞在聚酯薄膜圆盘上进行分离。将圆盘暴露于抗原中，并与淋巴细胞一起在微量滴定板的孔中孵育。该方法在技术上很简单，且不需要昂贵的材料。

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本文引用的文献

1
SEPARATION OF LYMPHOCYTES, POLYMORPHONUCLEAR LEUKOCYTES AND MONOCYTES ON GLASS COLUMNS, INCLUDING TISSUE CULTURE OBSERVATIONS.玻璃柱上淋巴细胞、多形核白细胞和单核细胞的分离，包括组织培养观察
Blood. 1964 Jun;23:811-28.
2
A quantitative method for studying tumour cells in blood.一种研究血液中肿瘤细胞的定量方法。
Lancet. 1961 Apr 22;1(7182):852-3. doi: 10.1016/s0140-6736(61)90177-5.
3
Macrophage-lymphocyte interaction in the antigen-induced blastogenic response of human peripheral blood leukocytes.巨噬细胞与淋巴细胞在人外周血白细胞抗原诱导的增殖反应中的相互作用。
J Immunol. 1968 Jun;100(6):1184-94.
4
The interaction of human monocytes and lymphocytes.人类单核细胞与淋巴细胞的相互作用。
J Exp Med. 1968 Dec 1;128(6):1309-25. doi: 10.1084/jem.128.6.1309.
5
Human monocytes and macrophages. Interaction with antigen and lymphocytes.人类单核细胞和巨噬细胞。与抗原及淋巴细胞的相互作用。
J Cell Biol. 1970 Jul;46(1):97-105. doi: 10.1083/jcb.46.1.97.
6
Precipitation of radioactively labeled samples: a semi-automatic multiple-sample processor.放射性标记样品的沉淀：一种半自动多样品处理器。
Cell Immunol. 1972 Jun;4(2):182-6. doi: 10.1016/0008-8749(72)90018-4.
7
Synergistic interaction of macrophages and lymphocytes in antigen-induced transformation of lymphocytes.巨噬细胞与淋巴细胞在抗原诱导的淋巴细胞转化中的协同相互作用。
J Exp Med. 1970 Jul 1;132(1):44-65. doi: 10.1084/jem.132.1.44.
8
Stimulation of subpopulations of human lymphocytes by a vaccine strain of Francisella tularensis.土拉弗朗西斯菌疫苗株对人淋巴细胞亚群的刺激作用。
Infect Immun. 1978 Jun;20(3):698-704. doi: 10.1128/iai.20.3.698-704.1978.
9
Function of macrophages in genetic control of immune responsiveness.巨噬细胞在免疫反应性遗传控制中的作用。
Fed Proc. 1978 Jan;37(1):79-85.
10
Signals regulating in vitro activation of lymphocytes.
Prog Allergy. 1976;20:65-194. doi: 10.1159/000398282.