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研究单核细胞在淋巴细胞刺激中作用的显微技术

Microtechnique for studies on the role of monocytes in the stimulation of lymphocytes.

作者信息

Tärnvik A

出版信息

Infect Immun. 1979 Jun;24(3):589-92. doi: 10.1128/iai.24.3.589-592.1979.

Abstract

A microtiter technique was used to measure lymphocyte stimulation by antigen-pulsed monocytes. Monocytes from human blood were isolated on polyester film disks. The disks were exposed to antigen and incubated together with lymphocytes in the wells of a microtiter plate. The method was technically simple and required no expensive materials.

摘要

采用微量滴定技术来测定经抗原刺激的单核细胞对淋巴细胞的刺激作用。用人血中的单核细胞在聚酯薄膜圆盘上进行分离。将圆盘暴露于抗原中,并与淋巴细胞一起在微量滴定板的孔中孵育。该方法在技术上很简单,且不需要昂贵的材料。

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本文引用的文献

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Signals regulating in vitro activation of lymphocytes.
Prog Allergy. 1976;20:65-194. doi: 10.1159/000398282.

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