Li Kang, Cheng Yan, Fang Chuanying
Hainan Yazhou Bay Seed Laboratory, Scool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya, China.
School of Tropical Agriculture and Forestry, Hainan University, Haikou, China.
Front Plant Sci. 2023 Dec 7;14:1322463. doi: 10.3389/fpls.2023.1322463. eCollection 2023.
Strigolactone (SL) plays essential roles in plant development and the metabolism of rice leaves. However, the impact of SL on the accumulation of nutritional metabolites in polished rice, as well as the transcription factors directly involved in SL synthesis, remains elusive. In this study, we performed a metabolome analysis on polished rice samples from mutants of an SL biosynthetic gene, (). Compared with those in the wild type plants, primary and secondary metabolites exhibited a series of alterations in the mutants. Notably, the mutants showed a substantial increase in the amino acids and vitamins content. Through a yeast one-hybridization screening assay, we identified OsSPL3 as a transcription factor that binds to the promoter, thereby inhibiting transcription and . Furthermore, we conducted a metabolic profiling analysis in polished rice from plants that overexpressed and observed enhanced levels of amino acids and vitamins. This study identified a novel transcriptional repressor of the SL biosynthetic gene and elucidated the regulatory roles of OsSPL3 and on the accumulation of nutritional metabolites in polished rice.
独脚金内酯（SL）在植物发育和水稻叶片代谢中起着至关重要的作用。然而，SL对精米中营养代谢物积累的影响，以及直接参与SL合成的转录因子，仍不清楚。在本研究中，我们对SL生物合成基因（）突变体的精米样本进行了代谢组分析。与野生型植物相比，初级和次级代谢物在突变体中表现出一系列变化。值得注意的是，突变体的氨基酸和维生素含量大幅增加。通过酵母单杂交筛选试验，我们鉴定出OsSPL3是一种与启动子结合的转录因子，从而抑制转录和。此外，我们对过表达的植物的精米进行了代谢谱分析，观察到氨基酸和维生素水平有所提高。本研究鉴定了一种新的SL生物合成基因转录抑制因子，并阐明了OsSPL3和对精米中营养代谢物积累的调控作用。
