Zhu Gaowei, Zhang Rongyan, Xie Qianyang, Li Peilun, Wang Fujun, Wang Lu, Li Chaojing
Key Laboratory of Textile Science & Technology Ministry of Education, College of Textiles, Donghua University, Shanghai, 201620, China.
Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, and Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, No. 639, Zhizaoju Rd., Shanghai, 200011, China.
Mater Today Bio. 2023 Nov 28;23:100880. doi: 10.1016/j.mtbio.2023.100880. eCollection 2023 Dec.
Biopolymer grafts often have limited biocompatibility, triggering excessive inflammatory responses similar to foreign bodies. Macrophage phenotype shifts are pivotal in the inflammatory response and graft success. The effects of the morphology and physical attributes of the material itself on macrophage polarization should be the focus. In this study, we prepared electrospun fibers with diverse diameters and formed a shish-kebab (SK) structure on the material surface by solution-induced crystallization, forming electrospun fiber scaffolds with diverse pore sizes and roughness. cell culture experiments demonstrated that SK structure fibers could regulate macrophage differentiation toward M2 phenotype, and the results of simulation of tissue reconstruction by the microenvironment demonstrated that the paracrine role of M2 phenotype macrophages could promote bone marrow mesenchymal stem cells (BMSCs) to differentiate into osteoblasts. In rats implanted with a subcutaneous SK-structured fiber scaffold, the large-pore size and low-stiffness SK fiber scaffolds demonstrated superior immune performance, less macrophage aggregation, and easier differentiation to the anti-inflammatory M2 phenotype. Large pore sizes and low-stiffness SK fiber scaffolds guide the morphological design of biological scaffolds implanted , which is expected to be an effective strategy for reducing inflammation when applied to graft materials in clinical settings.
生物聚合物移植物通常具有有限的生物相容性，会引发类似于异物的过度炎症反应。巨噬细胞表型转变在炎症反应和移植物成功中起关键作用。材料本身的形态和物理属性对巨噬细胞极化的影响应成为研究重点。在本研究中，我们制备了具有不同直径的电纺纤维，并通过溶液诱导结晶在材料表面形成了串珠状（SK）结构，从而形成了具有不同孔径和粗糙度的电纺纤维支架。细胞培养实验表明，SK结构纤维可调节巨噬细胞向M2表型分化，微环境组织重建模拟结果表明，M2表型巨噬细胞的旁分泌作用可促进骨髓间充质干细胞（BMSC）分化为成骨细胞。在植入皮下SK结构纤维支架的大鼠中，大孔径和低刚度的SK纤维支架表现出卓越的免疫性能、较少的巨噬细胞聚集以及更容易分化为抗炎性M2表型。大孔径和低刚度的SK纤维支架指导了植入生物支架的形态设计，有望成为临床应用于移植材料时减轻炎症的有效策略。
