Zheng Wei, Xi Jiahui, Zi Yin, Wang Jinling, Chi Yue, Chen Min, Zou Qingjian, Tang Chengcheng, Zhou Xiaoqing
Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Biotechnology and Health Science, Wuyi University, Jiangmen, 529000, China.
Vet World. 2023 Nov;16(11):2374-2381. doi: 10.14202/vetworld.2023.2374-2381. Epub 2023 Nov 27.
African swine fever (ASF), a globally transmitted viral disease caused by ASF virus (ASFV), can severely damage the global trade economy. Laboratory diagnostic methods, including pathogen and serological detection techniques, are currently used to monitor and control ASF. Because the large double-stranded DNA genome of the mature virus particle is wrapped in a membrane, the stability of ASFV and its genome is maintained in most natural environments. This study aimed to investigate the stability of ASFV under different environmental conditions from both genomic and antibody perspectives, and to provide a theoretical basis for the prevention and elimination of ASFV.
In this study, we used quantitative real-time polymerase chain reaction for pathogen assays and enzyme-linked immunosorbent assay for serological assays to examine the stability of the ASFV genome and antibody, respectively, under different environmental conditions.
The stability of the ASFV genome and antibody under high-temperature conditions depended on the treatment time. In the pH test, the ASFV genome and antibody remained stable in both acidic and alkaline environments. Disinfection tests revealed that the ASFV genome and antibody were susceptible to standard disinfection methods.
Collectively, the results demonstrated that the ASFV genome is highly stable in favorable environments but are also susceptible to standard disinfection methods. This study focuses on the stability of the ASFV genome under different conditions and provides various standard disinfection methods for the prevention and control of ASF.
非洲猪瘟（ASF）是由非洲猪瘟病毒（ASFV）引起的一种全球传播的病毒性疾病，会严重损害全球贸易经济。目前，包括病原体和血清学检测技术在内的实验室诊断方法被用于监测和控制ASF。由于成熟病毒粒子的大型双链DNA基因组被包裹在膜中，ASFV及其基因组在大多数自然环境中都能保持稳定。本研究旨在从基因组和抗体两个角度研究ASFV在不同环境条件下的稳定性，为预防和根除ASFV提供理论依据。
在本研究中，我们分别使用定量实时聚合酶链反应进行病原体检测，以及酶联免疫吸附测定进行血清学检测，以检测ASFV基因组和抗体在不同环境条件下的稳定性。
高温条件下ASFV基因组和抗体的稳定性取决于处理时间。在pH测试中，ASFV基因组和抗体在酸性和碱性环境中均保持稳定。消毒测试表明，ASFV基因组和抗体对标准消毒方法敏感。
总体而言，结果表明ASFV基因组在适宜环境中高度稳定，但也对标准消毒方法敏感。本研究关注了不同条件下ASFV基因组的稳定性，并为ASF的防控提供了多种标准消毒方法。
