Sachivkina Nadezhda, Karamyan Arfenya, Petrukhina Olesya, Kuznetsova Olga, Neborak Ekaterina, Ibragimova Alfia
Department of Microbiology V.S. Kiktenko, Institute of Medicine, Peoples' Friendship University of Russia (RUDN University), Moscow, Russia.
Department of Veterinary Medicine, Agrarian Technological Institute, Peoples' Friendship University of Russia (RUDN University), Moscow, Russia.
Vet World. 2023 Nov;16(11):2192-2199. doi: 10.14202/vetworld.2023.2192-2199. Epub 2023 Nov 1.
Fungal infections are a growing problem for both humans and animals due to the emergence of pathogenic strains resistant to modern antifungal treatments. To evaluate the efficacy of new antifungal drugs, it is essential to develop animal models that demonstrate typical responses to both the infection (pathogenesis and clinical course) and to the treatment, including adverse effects. In this study, we established a rabbit otitis model by infection of an aggressive multidrug-resistant strain from dogs, C23, with no need for concomitant immunosuppression.
Twenty healthy adult male gray giant rabbits (1 year old, 5.5 kg) were inoculated once with C23 at 10 colony-forming units/mL. We observed the clinical signs of the disease and collected ear smears and blood samples every 5 days.
The infection progressed rapidly and exhibited characteristic clinical signs without spontaneous recovery for at least 1 month. In fact, substantial deterioration was observed as evidenced by blood parameters.
This rabbit otitis model established using an aggressive drug-resistant fungus strain without immunosuppression could prove valuable for testing novel antifungal agents.
由于对现代抗真菌治疗产生耐药性的致病菌株的出现，真菌感染对人类和动物来说都是一个日益严重的问题。为了评估新型抗真菌药物的疗效，开发能够展示对感染（发病机制和临床病程）以及治疗（包括不良反应）的典型反应的动物模型至关重要。在本研究中，我们通过感染来自犬类的具有侵袭性的多重耐药菌株C23建立了兔中耳炎模型，无需同时进行免疫抑制。
20只健康成年雄性灰色巨型兔（1岁，5.5千克）以10个菌落形成单位/毫升的浓度接种一次C23。我们观察疾病的临床症状，并每5天收集耳部涂片和血液样本。
感染进展迅速，呈现出特征性临床症状，至少1个月内未出现自发恢复。事实上，血液参数显示病情有显著恶化。
这种使用具有侵袭性的耐药真菌菌株且无需免疫抑制建立的兔中耳炎模型，可能对测试新型抗真菌药物具有重要价值。
