Davis H M, Hines H B, Edwards J R
Carbohydr Res. 1986 Nov 15;156:69-77. doi: 10.1016/s0008-6215(00)90100-9.
The structure of a water-insoluble polysaccharide produced by the D-glucosyl-transferase of Streptococcus mutans 6715 has been elucidated through periodate oxidation, Smith degradation, dextranase digestion, concanavalin A binding studies, and methylation combined with g.l.c.-m.s. analysis. These studies show that the D-glucan is comprised of 67% alpha-(1----3) linkages in a contiguous backbone with the remaining 33% as alpha-(1----6) linkages, possibly as linear residues extending from alpha-(1----6) branch points. Of the residues, 14% are branch points and the ratio of linear alpha-(1----3) residues in the backbone to alpha-(1----6) residues in the side chain was found to be 5:2. Dextranase digestion and Smith degradation both gave rise to a high-molecular-weight fraction that is only alpha-(1----3) linked.
通过高碘酸盐氧化、史密斯降解、葡聚糖酶消化、伴刀豆球蛋白A结合研究以及甲基化结合气相色谱-质谱分析，已阐明变形链球菌6715的D-葡萄糖基转移酶产生的一种水不溶性多糖的结构。这些研究表明，该D-葡聚糖在连续主链中由67%的α-(1→3)键组成，其余33%为α-(1→6)键，可能是从α-(1→6)分支点延伸的线性残基。在这些残基中，14%是分支点，并且发现主链中线性α-(1→3)残基与侧链中α-(1→6)残基的比例为5:2。葡聚糖酶消化和史密斯降解均产生了仅由α-(1→3)连接的高分子量级分。
