Department of Evolution, Ecology, and Organismal Biology, University of California Riverside, Riverside, CA 92521, USA.
Department of Biology, Duke University, Durham, NC 27708, USA.
G3 (Bethesda). 2024 Mar 6;14(3). doi: 10.1093/g3journal/jkad296.
Penstemon is the most speciose flowering plant genus endemic to North America. Penstemon species' diverse morphology and adaptation to various environments have made them a valuable model system for studying evolution. Here, we report the first full reference genome assembly and annotation for Penstemon davidsonii. Using PacBio long-read sequencing and Hi-C scaffolding technology, we constructed a de novo reference genome of 437,568,744 bases, with a contig N50 of 40 Mb and L50 of 5. The annotation includes 18,199 gene models, and both the genome and transcriptome assembly contain over 95% complete eudicot BUSCOs. This genome assembly will serve as a valuable reference for studying the evolutionary history and genetic diversity of the Penstemon genus.
风铃草属是北美特有的最具物种多样性的开花植物属。风铃草属物种形态多样，适应各种环境，使它们成为研究进化的有价值的模式系统。在这里，我们报告了第一个风铃草属全参考基因组组装和注释。使用 PacBio 长读测序和 Hi-C 支架技术，我们构建了一个从头参考基因组，大小为 437,568,744 个碱基，contig N50 为 40Mb，L50 为 5。注释包括 18,199 个基因模型，基因组和转录组组装都包含超过 95%完整的真双子叶植物 BUSCOs。这个基因组组装将作为研究风铃草属进化历史和遗传多样性的宝贵参考。
