Yucesan Banucicek, Ozkan Ozcan, Tuncer Sinem, Ocal Zekeriya
Department of Healthcare Management, Faculty of Health Science, Cankırı Karatekin University, Cankırı, Turkiye.
Department of Biology, Faculty of Sience, Cankırı Karatekin University, Çankırı, Turkiye.
Iran J Parasitol. 2023 Oct-Dec;18(4):483-493. doi: 10.18502/ijpa.v18i4.14257.
Canin leishmaniasis (CanL), mostly caused by , is one of the most important vector-borne diseases in dogs in the Mediterranean region. In this study, we aimed to determine the disease profile in this region by firstly making microscopic and then molecular analyzes in the samples taken from the dogs.
Overall, 112 whole blood samples taken from dogs for clinical applications by a veterinarian in Cankırı between December 2021 and November 2022 were used. After blood collection, both thin and thick drop blood smear preparations were prepared and evaluated for Giemsa staining. was investigated by Real time-PCR (RT-PCR) method from all blood samples. Sequence analysis and phylogenetic tree study were performed on positive samples.
Both microscopic and RT-PCR analyzes were performed. In both studies, 3 of the 112 samples were positive. Because of the sequence analysis, they were Sequence analysis was performed from the samples found 3 positive. The phylogenetic tree was drawn by making NCBI (National Center for Biotechnology) data entries of the positive samples (Accession numbers: OQ184728, OQ184729, OQ184730).
Dogs are important, as they are reservoir of this disease. In this study, 3 (2.7%) positive Leishmaniasis was detected in dogs in Cankırı. Ultimately, this should prompt discussion about new strategies going forward to combat infection caused by
犬利什曼病（CanL）主要由[病原体未提及]引起，是地中海地区犬类最重要的媒介传播疾病之一。在本研究中，我们旨在通过首先对从犬只采集的样本进行显微镜检查，然后进行分子分析，来确定该地区的疾病概况。
总共使用了2021年12月至2022年11月期间在坎基尔由一名兽医为临床应用从犬只采集的112份全血样本。采血后，制备薄血涂片和厚血涂片并进行吉姆萨染色评估。对所有血样采用实时荧光定量聚合酶链反应（RT-PCR）方法检测[检测对象未提及]。对阳性样本进行序列分析和系统发育树研究。
进行了显微镜检查和RT-PCR分析。在两项研究中，112份样本中有3份呈阳性。由于进行了序列分析，它们是[具体序列未提及]。对3份呈阳性的样本进行了序列分析。通过将阳性样本的数据录入美国国立生物技术信息中心（NCBI，登录号：OQ184728、OQ184729、OQ184730）绘制了系统发育树。
犬类作为这种疾病的储存宿主很重要。在本研究中，在坎基尔的犬类中检测到3例（2.7%）阳性利什曼病。最终，这应该促使人们讨论未来对抗由[感染源未提及]引起的感染的新策略。
