Guo Ning, Raincrow Jeremy, Chiu Chi-Hua, Yu Lei
Department of Genetics, Rutgers University, Piscataway, NJ, 08854, USA.
School of Biomedical Sciences, and Department of Biological Sciences, Kent State University, Kent, Ohio, 44242, USA.
Biochem Biophys Rep. 2024 Jan 1;37:101631. doi: 10.1016/j.bbrep.2023.101631. eCollection 2024 Mar.
SIP30, characterized by a coiled-coil functional domain, plays a key role in regulating synaptic vesicle exocytosis and is implicated in neuropathic pain resulting from peripheral nerve injury. Because neuropathic pain is studied in primates (including human), domesticated animals, and rodents, we conducted a phylogenetic analysis of SIP30 in selected species of these three groups of mammals. SIP30 exhibits a high degree of sequence divergence in comparison to its protein binding partners SNAP25 and ZW10, which show broad sequence conservation. Notably, we observed an increased rate of change in the highly conserved coiled-coil domain in the SIP30 protein, specifically within primates. This observation suggests an accelerated adaptation of this functional domain in primate species.
SIP30具有卷曲螺旋功能结构域，在调节突触小泡胞吐作用中起关键作用，并与周围神经损伤引起的神经性疼痛有关。由于在灵长类动物（包括人类）、家养动物和啮齿动物中对神经性疼痛进行了研究，我们对这三类哺乳动物的选定物种中的SIP30进行了系统发育分析。与显示广泛序列保守性的蛋白质结合伙伴SNAP25和ZW10相比，SIP30表现出高度的序列差异。值得注意的是，我们观察到SIP30蛋白中高度保守的卷曲螺旋结构域的变化速率增加，特别是在灵长类动物中。这一观察结果表明该功能结构域在灵长类物种中加速适应。
