Shanghai Institute of Phage, Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, China.
Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, 130062, China.
Virus Genes. 2020 Aug;56(4):498-507. doi: 10.1007/s11262-020-01766-0. Epub 2020 May 25.
Acinetobacter pittii is an important pathogen causing nosocomial infection worldwide. In this study, a multidrug-resistant A. pittii ABC38 was used as host bacterium to isolate the lytic phage vB_ApiP_XC38. The biological characteristics of vB_ApiP_XC38 were studied and the genome was sequenced and analyzed. vB_ApiP_XC38 belonged to Podoviridae family. The phage had double-stranded genome, which comprised 79,328 bp with 39.58% G+C content displaying very low similarity (< 1% identity) with published genomes of other phages and bacteria. A total of 97 open reading frames (ORFs) were predicted and contained nucleotide metabolism and replication module, structural components module, and lysis module. The ANI, AAI, and phylogenetic analysis indicated that all phages were found distant from vB_ApiP_XC38. Altogether, morphological, genomics, and phylogenetic analysis suggest that vB_ApiP_XC38 is more likely a novel phage of A. pittii.
鲍曼不动杆菌是一种重要的引起全球医院感染的病原体。在本研究中，以一株多药耐药的鲍曼不动杆菌 ABC38 为宿主菌，分离出溶菌噬菌体 vB_ApiP_XC38。研究了 vB_ApiP_XC38 的生物学特性，并对其基因组进行了测序和分析。vB_ApiP_XC38 属于 Podoviridae 科。噬菌体具有双链基因组，由 79328 bp 组成，G+C 含量为 39.58%，与已发表的其他噬菌体和细菌基因组的相似度非常低（<1%同一性）。共预测到 97 个开放阅读框（ORFs），包含核苷酸代谢和复制模块、结构成分模块和裂解模块。ANI、AAI 和系统发育分析表明，所有噬菌体与 vB_ApiP_XC38 均相距较远。综上所述，形态学、基因组学和系统发育分析表明，vB_ApiP_XC38 极有可能是一种新的鲍曼不动杆菌噬菌体。
