Samal S K, Dopazo C P, McPhillips T H, Baya A, Mohanty S B, Hetrick F M
College of Veterinary Medicine, University of Maryland, College Park 20742.
J Virol. 1990 Nov;64(11):5235-40. doi: 10.1128/JVI.64.11.5235-5240.1990.
The characteristics of a rotaviruslike (SBR) virus isolated from striped bass (Morone saxatilis) were examined following purification of viruses from infected cell cultures. Virions had a double-layered capsid of icosahedral symmetry and a diameter of 75 nm. Purified viruses contained five polypeptides ranging in molecular mass from 130 to 35 kDa. None of the structural proteins were glycosylated. Treatment with EDTA did not remove the outer capsid. By using enzymes and a chaotropic agent, it was shown that VP5 was the most external polypeptide. The genome of SBR virus was composed of 11 segments of double-stranded RNA (dsRNA). The electrophoretic pattern of the dsRNA of SBR virus was different from that of reovirus type 1 (Lang) and rotavirus (SA11) dsRNA. The SBR virus was compared with reovirus type 1 and SA11 virus by RNA-RNA blot hybridization. There was no cross-hybridization between any of the genome segments of the SBR, reovirus type 1, or SA11 viruses. Antigenic comparison of SBR virus and SA11 virus by cross-immunoprecipitation and cross-immunofluorescence tests did not show any relationship. These results suggest that SBR virus could represent a new genus within the family Reoviridae.
从条纹鲈（Morone saxatilis）中分离出的一种类轮状病毒（SBR）在从感染的细胞培养物中纯化病毒后，对其特性进行了检测。病毒粒子具有二十面体对称的双层衣壳，直径为75纳米。纯化的病毒含有五种分子量在130至35千道尔顿之间的多肽。没有一种结构蛋白是糖基化的。用乙二胺四乙酸（EDTA）处理不能去除外衣壳。通过使用酶和一种离液剂，表明VP5是最外层的多肽。SBR病毒的基因组由11个双链RNA（dsRNA）片段组成。SBR病毒的dsRNA电泳图谱与呼肠孤病毒1型（Lang）和轮状病毒（SA11）的dsRNA不同。通过RNA-RNA印迹杂交将SBR病毒与呼肠孤病毒1型和SA11病毒进行了比较。SBR、呼肠孤病毒1型或SA11病毒的任何基因组片段之间均无交叉杂交。通过交叉免疫沉淀和交叉免疫荧光试验对SBR病毒和SA11病毒进行抗原比较，未显示出任何关系。这些结果表明，SBR病毒可能代表呼肠孤病毒科中的一个新属。
