Bergh A, Rooth P, Widmark A, Damber J E
J Reprod Fertil. 1987 Jan;79(1):135-43. doi: 10.1530/jrf.0.0790135.
Adult rats were injected subcutaneously with 50 i.u. hCG and vascular permeability was compared to that in saline-treated control rats by two independent methods. At 4 h after hCG treatment the rats were injected intra-arterially (i.a.) with FITC-labelled macromolecular dextran (Mr 150,000) and the testicular microcirculation was studied in vivo by using a fluorescence microscope. Other rats were injected i.a. with a suspension of colloidal carbon and the location of leaking blood vessels was recorded in sections from the testes by light and electron microscopy. In hCG-treated animals leucocytes were found adhering to the endothelium in post-capillary venules and in these venular segments dextran was leaking into the interstitium. Carbon particles were deposited in the walls of post-capillary venules and leucocytes migrated through open interendothelial cell gaps in hCG-treated animals. In control animals leucocyte adhesion and migration were not observed, the injected dextran remained in the circulation and the blood vessels were not labelled by carbon. It is suggested that the hCG-induced increase in testicular interstitial fluid volume, like the tissue oedema in inflammation, is caused by a leucocyte-mediated increase in venular permeability.
成年大鼠皮下注射50国际单位的人绒毛膜促性腺激素（hCG），并通过两种独立的方法将血管通透性与生理盐水处理的对照大鼠进行比较。在hCG处理后4小时，给大鼠动脉内注射异硫氰酸荧光素（FITC）标记的大分子右旋糖酐（分子量150,000），并使用荧光显微镜在体内研究睾丸微循环。其他大鼠动脉内注射胶体碳悬液，并通过光学显微镜和电子显微镜记录睾丸切片中血管渗漏的位置。在hCG处理的动物中，发现白细胞粘附于毛细血管后微静脉的内皮，在这些微静脉段中，右旋糖酐渗漏到间质中。在hCG处理的动物中，碳颗粒沉积在毛细血管后微静脉壁上，白细胞通过开放的内皮细胞间隙迁移。在对照动物中未观察到白细胞粘附和迁移，注射的右旋糖酐保留在循环中，血管未被碳标记。有人提出，hCG诱导的睾丸间质液体积增加，如同炎症中的组织水肿一样，是由白细胞介导的微静脉通透性增加所引起的。
