Logigan Corina-Lenuța, Delaite Christelle, Popa Marcel, Băcăiță Elena Simona, Tiron Crina Elena, Peptu Cristian, Peptu Cătălina Anișoara
Department of Natural and Synthetic Polymers, Faculty of Chemical Engineering and Environmental Protection "Cristofor Simionescu", "Gheorghe Asachi" Technical University of Iasi, Bld. Prof. Dr. Doc. Dimitrie Mangeron Street, No. 73, 700050 Iasi, Romania.
Laboratory of Photochemistry and Macromolecular Engineering, Institute J.B. Donnet, University of Haute Alsace, 68100 Mulhouse, France.
Polymers (Basel). 2024 Jan 2;16(1):144. doi: 10.3390/polym16010144.
Nanotechnology is the science of creating materials at the nanoscale by using various devices, structures, and systems that are often inspired by nature. Micro- and nanoparticles (MPs, NPs) are examples of such materials that have unique properties and can be used as carriers for delivering drugs for different biomedical applications. Chitosan (CS) is a natural polysaccharide that has been widely studied, but it has a problem with low water solubility at neutral or basic pH, which limits its processability. The goal of this work was to use a chemically modified CS with poly(ethylene glycol) methyl ether acrylate (PEGA) to prepare CS micronic and submicronic particles (MPs/NPs) that can deliver different types of antibiotics, respectively, levofloxacin (LEV) and Ciprofloxacin (CIP). The particle preparation procedure employed a double crosslinking method, ionic followed by a covalent, in a water/oil emulsion. The studied process parameters were the precursor concentration, stirring speeds, and amount of ionic crosslinking agent. MPs/NPs were characterized by FT-IR, SEM, light scattering granulometry, and Zeta potential. MPs/NPs were also tested for their water uptake capacity in acidic and neutral pH conditions, and the results showed that they had a pH-dependent behavior. The MPs/NPs were then used to encapsulate two separate drugs, LEV and CIP, and they showed excellent drug loading and release capacity. The MPs/NPs were also found to be safe for cells and blood, which demonstrated their potential as suitable drug delivery systems for biomedical applications.
纳米技术是一门通过使用各种通常受自然启发的设备、结构和系统来制造纳米级材料的科学。微米和纳米颗粒（MPs、NPs）就是这类具有独特性质的材料的例子，它们可用作载体，用于不同生物医学应用中的药物递送。壳聚糖（CS）是一种已被广泛研究的天然多糖，但它在中性或碱性pH条件下存在水溶性低的问题，这限制了其可加工性。这项工作的目标是使用聚（乙二醇）甲基醚丙烯酸酯（PEGA）对CS进行化学改性，以制备分别可递送不同类型抗生素左氧氟沙星（LEV）和环丙沙星（CIP）的CS微米和亚微米颗粒（MPs/NPs）。颗粒制备过程采用双交联方法，在水/油乳液中先进行离子交联，然后进行共价交联。研究的工艺参数包括前驱体浓度、搅拌速度和离子交联剂的用量。通过傅里叶变换红外光谱（FT-IR）、扫描电子显微镜（SEM）、光散射粒度分析和Zeta电位对MPs/NPs进行了表征。还测试了MPs/NPs在酸性和中性pH条件下的吸水能力，结果表明它们具有pH依赖性行为。然后将MPs/NPs用于包封两种不同的药物LEV和CIP，它们表现出优异的载药和释药能力。还发现MPs/NPs对细胞和血液安全，这证明了它们作为生物医学应用中合适药物递送系统的潜力。
