Sericultural Research Institute, Zhejiang Academy of Agricultural Science, Hangzhou, China.
Pharmacology and Toxicology Research Center, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
PLoS One. 2018 Oct 5;13(10):e0205007. doi: 10.1371/journal.pone.0205007. eCollection 2018.
Phellinus igniarius, which is called Sanghuang in Chinese, is a fungal herb widely used in Traditional Chinese Medicine to treat stomachache, inflammation and tumors. Recent studies have demonstrated the antitumor, anti-diabetic, anti-inflammatory and immunity-modulating activities of P. igniarius. In the present study, we investigated that ameliorating effect of the aqueous extract of P. igniarius fruiting body (SH) on dextran sodium sulfate (DSS)-induced colitis in C57BL/6 mice. Treatment with SH (250 and 400 mg/kg) for 8 weeks effectively alleviated the pathological indicators of colitis such as bodyweight reduction, disease activity index score, shortening of colon length and abnormal colon histology. The plasma levels of lipopolysaccharide (LPS) and inflammatory factors such as interleukin-6 (IL-6), IL-1β and tumor necrosis factor (TNF)-α were all significantly reduced. Supplementation of SH (10 mg/L) also inhibited LPS-elicited IL-1β production by RAW264.7 macrophages. Real-time PCR and western blot showed that treatment with SH significantly inhibited the phosphorylation of nuclear factor kappa B inhibitor alpha (IκBα) and decreased the expression of IL-6/IL-1β-maturation genes such as apoptosis-associated speck-like protein (ASC3) and caspase-1 in the colon of DSS-induced colitis mice. These results suggest that SH is adequate for the treatment of colitis. Inhibiting the expression and release of inflammatory factors may participate in the colitis-ameliorating effect of SH.
桑黄，又称火木层孔菌，是一种真菌草药，在中国传统医学中被广泛用于治疗胃痛、炎症和肿瘤。最近的研究表明，火木层孔菌具有抗肿瘤、抗糖尿病、抗炎和免疫调节活性。在本研究中，我们研究了火木层孔菌子实体水提物（SH）对葡聚糖硫酸钠（DSS）诱导的 C57BL/6 小鼠结肠炎的改善作用。8 周的 SH（250 和 400 mg/kg）治疗有效缓解了结肠炎的病理指标，如体重减轻、疾病活动指数评分、结肠缩短和异常结肠组织学。血浆中脂多糖（LPS）和炎症因子如白细胞介素-6（IL-6）、白细胞介素-1β（IL-1β）和肿瘤坏死因子（TNF）-α的水平均显著降低。SH（10 mg/L）的补充也抑制了 LPS 诱导的 RAW264.7 巨噬细胞中 IL-1β的产生。实时 PCR 和 Western blot 显示，SH 治疗显著抑制了核因子 kappa B 抑制剂 alpha（IκBα）的磷酸化，并降低了 DSS 诱导的结肠炎小鼠结肠中 IL-6/IL-1β成熟基因如凋亡相关斑点样蛋白（ASC3）和半胱天冬酶-1的表达。这些结果表明，SH 足以治疗结肠炎。抑制炎症因子的表达和释放可能参与了 SH 的结肠炎改善作用。
