Chen Shuxin, Liu Yue, Qin Zhiyu, Wen Guiqing, Jiang Zhiliang
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin 541004, China.
Guangxi Key Laboratory of Environmental Processes and Remediation in Ecologically Fragile Regions, Guilin, 541004, China.
Analyst. 2024 Feb 12;149(4):1179-1189. doi: 10.1039/d3an01902k.
Malachite green (MG) is highly toxic, persistent, and carcinogenic, and its widespread use is a danger to the ecosystem and a threat to public health and food safety, making it necessary to develop new sensitive multimode molecular spectroscopy methods. In this work, a new copper-based nanomaterial (CuNM) was prepared by a high-temperature roasting using a copper metal-organic framework (CuMOF) as precursor. The as-prepared CuNM was characterized using X-ray diffraction (XRD), X-ray photoelectron spectroscopy, transmission electron microscopy (TEM), and BET surface area analysis. CuNM was found to catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by HO to produce the oxidation product TMBOX; however, subsequently, the MG aptamer (Apt) could be adsorbed on the CuNM surface by intermolecular interaction, which would inhibit the catalytic performance. After the addition of MG to be tested, the CuNM previously adsorbed by the Apt was transformed into its free state, thus restoring its catalytic activity. This new nanocatalytic indicator reaction could be monitored by surface-enhanced Raman scattering (SERS)/resonance Rayleigh scattering (RRS)/fluorescence (FL)/absorption (Abs) quadruple-mode methods. The SERS determination range was 0.004-0.4 nmol L MG, with a limit of detection of 0.0032 nM. In this way, a rapid, stable, and sensitive method for the determination of MG residues in the environment was established.
孔雀石绿（MG）具有高毒性、持久性和致癌性，其广泛使用对生态系统构成威胁，对公众健康和食品安全造成危害，因此有必要开发新的灵敏多模式分子光谱方法。在本工作中，以铜基金属有机框架（CuMOF）为前驱体，通过高温焙烧制备了一种新型铜基纳米材料（CuNM）。采用X射线衍射（XRD）、X射线光电子能谱、透射电子显微镜（TEM）和BET比表面积分析对所制备的CuNM进行了表征。发现CuNM能催化HO氧化3,3',5,5'-四甲基联苯胺（TMB）生成氧化产物TMBOX；然而，随后MG适配体（Apt）可通过分子间相互作用吸附在CuNM表面，这会抑制其催化性能。加入待测MG后，先前被Apt吸附的CuNM转变为游离状态，从而恢复其催化活性。这种新型纳米催化指示反应可通过表面增强拉曼散射（SERS）/共振瑞利散射（RRS）/荧光（FL）/吸收（Abs）四模式方法进行监测。SERS测定范围为0.004 - 0.4 nmol L MG，检测限为0.0032 nM。通过这种方式，建立了一种快速、稳定且灵敏的环境中MG残留量测定方法。
