Han Shunping, Al-Jamal Khuloud T
Institute of Pharmaceutical Science Faculty of Life Sciences & Medicine King's College London Franklin-Wilkins Building, 150 Stamford Street London SE1 9NH UK.
Part Part Syst Charact. 2023 Oct;40(10):2300043. doi: 10.1002/ppsc.202300043. Epub 2023 Sep 5.
Synthesizing gold nanorods (AuNRs) by seed-mediated growth method results in the presence of undesired size and shape particles by-products occupying 10-90% of the population. In this study, AuNRs are synthesized by the seed-mediated growth method using cetyltrimethylammonium bromide (CTAB) as a surfactant. AuNRs with redshifted longitudinal localized surface plasmon resonance (LLSPR) peak, localized in the biological "transparency window" (650-1350 nm), are synthesized after optimizing seed solution, silver nitrate solution, and hydrochloric acid solution volumes, based on the published protocols. A two-step purification method, dialysis followed by centrifugation, is applied to remove excess CTAB and collect LLSPR-redshifted AuNRs with high rod purity (>90%). CTAB is subsequently exchanged with polyethylene glycol (PEG) to improve AuNRs biocompatibility. PEGylated AuNRs are confirmed innocuous to both SN4741 cells and B16F10 cells by the modified MTT assay and the modified lactate dehydrogenase (LDH) assay up to 1 nm and 24 h incubation. In this study, a combined facile synthesis, purification, and surface functionalization approach is proposed to obtain water-dispersible monodispersed AuNRs for drug delivery applications.
通过种子介导生长法合成金纳米棒（AuNRs）会产生不需要的尺寸和形状的颗粒副产物，其占总体的10 - 90%。在本研究中，使用十六烷基三甲基溴化铵（CTAB）作为表面活性剂，通过种子介导生长法合成AuNRs。基于已发表的方案，在优化种子溶液、硝酸银溶液和盐酸溶液的体积后，合成了纵向局域表面等离子体共振（LLSPR）峰发生红移且位于生物“透明窗口”（650 - 1350 nm）的AuNRs。采用两步纯化方法，即先透析后离心，以去除过量的CTAB并收集棒纯度高（>90%）的LLSPR红移的AuNRs。随后用聚乙二醇（PEG）交换CTAB以提高AuNRs的生物相容性。通过改良的MTT法和改良的乳酸脱氢酶（LDH）法证实，经长达1 nm和24 h孵育后，聚乙二醇化的AuNRs对SN4741细胞和B16F10细胞均无毒害作用。在本研究中，提出了一种简便的合成、纯化和表面功能化相结合的方法，以获得用于药物递送应用的水分散性单分散AuNRs。
