Department of Biological Sciences, Ohio University, Athens, OH, USA.
Molecular and Cellular Biology Program, Ohio University, Athens, OH, USA.
Methods Mol Biol. 2024;2741:3-10. doi: 10.1007/978-1-0716-3565-0_1.
Investigations into the biological role and composition of bacterial extracellular vesicles have grown in popularity in recent years. Vesicles perform a variety of functions during interactions with eukaryotic host cells, ranging from antibiotic resistance to immune modulation. It is necessary to isolate vesicles in order to understand their biological functions. Here we describe a polymer-based precipitation method allowing high-yield isolation of extracellular vesicles and their cargo RNA from the Gram-positive bacterium Staphylococcus aureus.
近年来，对细菌细胞外囊泡的生物学作用和组成的研究越来越受到关注。囊泡在与真核宿主细胞相互作用过程中发挥多种功能，从抗生素耐药性到免疫调节。为了了解它们的生物学功能，有必要对囊泡进行分离。在这里，我们描述了一种基于聚合物的沉淀方法，可从革兰氏阳性菌金黄色葡萄球菌中高效分离细胞外囊泡及其货物 RNA。
