Yu Chengbing, Wang Di, Shen Cheng, Luo Zhen, Zhang Hongcai, Zhang Jing, Xu Weina, Xu Jianxiong
Shanghai Key Laboratory for Veterinary and Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Animals (Basel). 2024 Jan 6;14(2):190. doi: 10.3390/ani14020190.
This study aimed to investigate the dynamic changes in hepatic glucose metabolism in response to early weaning. A total of 60 piglets were randomly selected and weaned at 21 days old. Six piglets were slaughtered on the weaning day (d0) and at 1 (d1), 4 (d4), 7 (d7), and 14 (d14) days postweaning. The results illustrated that body weight significantly increased from d4 to d14 ( < 0.001). Serum glucose fell sharply after weaning and then remained at a low level from d1 to d14 ( < 0.001). Serum insulin decreased from d4 ( < 0.001), which caused hepatic glycogen to be broken down ( = 0.007). The glucose-6-phosphatase activity increased from d0 to d4 and then decreased from d4 to d14 ( = 0.039). The pyruvate carboxylase activity presented a significant sustained increase from d0 to d14 ( < 0.001). The succinate ( = 0.006) and oxaloacetate ( = 0.003) content on d4 was lower than that on d0. The succinate dehydrogenase activity ( = 0.008) and ATP ( = 0.016) production decreased significantly on d4 compared to that on d0. Taken together, these findings reveal the dynamic changes of metabolites and enzymes related to hepatic glycometabolism and the TCA (tricarboxylic acid) cycle in piglets after weaning. Our findings enrich weaning stress theory and might provide a reference for dietary intervention.
本研究旨在探讨早期断奶对肝脏葡萄糖代谢的动态影响。总共随机选取60头仔猪，在21日龄时断奶。在断奶当天（d0）以及断奶后1天（d1）、4天（d4）、7天（d7）和14天（d14）分别屠宰6头仔猪。结果表明，从d4到d14体重显著增加（<0.001）。断奶后血清葡萄糖急剧下降，然后从d1到d14维持在较低水平（<0.001）。血清胰岛素从d4开始下降（<0.001），这导致肝糖原分解（=0.007）。葡萄糖-6-磷酸酶活性从d0到d4升高，然后从d4到d14下降（=0.039）。丙酮酸羧化酶活性从d0到d14呈现显著持续升高（<0.001）。d4时琥珀酸（=0.006）和草酰乙酸（=0.003）含量低于d0时。与d0相比，d4时琥珀酸脱氢酶活性（=0.008）和ATP生成（=0.016）显著降低。综上所述，这些发现揭示了断奶后仔猪肝脏糖代谢和三羧酸（TCA）循环相关代谢物和酶的动态变化。我们的发现丰富了断奶应激理论，并可能为饮食干预提供参考。
