Zhou Miao, Wu Zichen, Deng Donghua, Wang Bin, Zhou Xiaoling, Zhou Bingyu, Wang Chunping, Zeng Yan
College of Animal Science and Technology, Hunan Agricultural University, Changsha, China.
Hunan Institute of Microbiology, Changsha, China.
Front Vet Sci. 2024 Aug 7;11:1436282. doi: 10.3389/fvets.2024.1436282. eCollection 2024.
Oxidative damage resulting from weaning stress significantly impacts the growth performance and health status of piglets. Taurine, a dietary antioxidant with diverse functions, was investigated in this study for its protective role against weaning stress-induced oxidative damage and its underlying mechanism. Forty 28-day-old male castrated weaned piglets were randomly assigned to four groups. The control group received the basal diet, while the experimental groups were fed the basal diet supplemented with 0.1, 0.2%, or 0.3% taurine over a 28-day period. , HO was utilized to induce oxidative damage to the jejunal mucosa of piglets via IPEC-J2 cells. The results demonstrated that taurine supplementation reduced the incidence of diarrhea in piglets compared to that in the control group ( < 0.05); the addition of 0.2 and 0.3% taurine led to increased average daily gain and improved feed conversion efficiency in weaned piglets, showing a linear dose-response correlation ( < 0.05). Taurine supplementation at 0.2 and 0.3% enhanced the activities of serum CAT and GSH-Px while decreasing the levels of serum NO, XOD, GSSG, and MDA ( < 0.05). Moreover, it significantly elevated the levels of GSS, Trx, POD, complex I, mt-nd5, and mt-nd6, enhancing superoxide anion scavenging capacity and the hydroxyl-free scavenging rate in the livers of weaned piglets while reducing NO levels in the liver ( < 0.05). Additionally, 0.2 and 0.3% taurine supplementation decreased serum IL-6 levels and elevated the concentrations of IgA, IgG, and IL-10 in weaned piglets ( < 0.05). The levels of occludin, claudin, and ZO-1 in the jejunum mucosa of weaned piglets increased with 0.2 and 0.3% taurine supplementation ( < 0.05). In IPEC-J2 cells, pretreatment with 25 mM taurine for 24 h enhanced the activities of SOD and CAT; reduced the MDA content; upregulated the mRNA expression of various genes, including , occludin, claudin-1, , and ; and reversed the oxidative damage induced by HO exposure ( < 0.05). Overall, the findings suggest that the inclusion of 2 and 3% taurine in the diet can enhance growth performance, reduce diarrhea rates, ameliorate oxidative stress and inflammation, and promote intestinal barrier function in weaned piglets.
断奶应激导致的氧化损伤显著影响仔猪的生长性能和健康状况。牛磺酸是一种具有多种功能的膳食抗氧化剂，本研究对其在抵抗断奶应激诱导的氧化损伤中的保护作用及其潜在机制进行了探究。40头28日龄的雄性去势断奶仔猪被随机分为四组。对照组饲喂基础日粮，而实验组在28天的时间里饲喂添加了0.1%、0.2%或0.3%牛磺酸的基础日粮。通过IPEC-J2细胞利用HO诱导仔猪空肠黏膜的氧化损伤。结果表明，与对照组相比，添加牛磺酸降低了仔猪腹泻的发生率（<0.05）；添加0.2%和0.3%的牛磺酸使断奶仔猪的平均日增重增加，饲料转化率提高，呈线性剂量反应关系（<0.05）。添加0.2%和0.3%的牛磺酸可提高血清CAT和GSH-Px的活性，同时降低血清NO、XOD、GSSG和MDA的水平（<0.05）。此外，它显著提高了断奶仔猪肝脏中GSS、Trx、POD、复合体I、mt-nd5和mt-nd6的水平，增强了超氧阴离子清除能力和羟自由基清除率，同时降低了肝脏中的NO水平（<0.05）。此外，添加0.2%和0.3%的牛磺酸可降低断奶仔猪血清IL-6水平，提高IgA、IgG和IL-10的浓度（<0.05）。添加0.2%和0.3%的牛磺酸可使断奶仔猪空肠黏膜中occludin、claudin和ZO-1的水平升高（<0.05）。在IPEC-J2细胞中，用25 mM牛磺酸预处理24小时可提高SOD和CAT的活性；降低MDA含量；上调包括occludin、claudin-1等多种基因的mRNA表达，并逆转HO暴露诱导的氧化损伤（<0.05）。总体而言，研究结果表明，在日粮中添加2%和3%的牛磺酸可以提高断奶仔猪的生长性能，降低腹泻率，改善氧化应激和炎症，并促进肠道屏障功能。
