Gong Changtian, Yang Jian, Zhang Xiping, Wei Zhun, Wang Xingyu, Huang Xinghan, Yu Ling, Guo Weichun
Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, China.
Center of Regenerative Medicine, Renmin Hospital of Wuhan University, Wuhan 430060, China.
Biomedicines. 2023 Dec 28;12(1):74. doi: 10.3390/biomedicines12010074.
Bone defect repair poses significant challenges in orthopedics, thereby increasing the demand for bone substitutes. Magnesium phosphate cements (MPCs) are widely used for bone defect repair because of their excellent mechanical properties and biodegradability. However, high crystallinity and uncontrolled magnesium ion (Mg) release limit the surface bioactivity of MPCs in bone regeneration. Here, we fabricate chondroitin sulfate (CS) as a surface coating via the lyophilization method, namely CMPC. We find that the CS coating is uniformly distributed and improves the mechanical properties of MPC through anionic electrostatic adsorption, while mediating degradation-related controlled ion release of Mg. Using a combination of in vitro and in vivo analyses, we show that the CS coating maintained cytocompatibility while increasing the cell adhesion area of MC3T3-E1s. Furthermore, we display accelerated osteogenesis and angiogenesis of CMPC, which are related to appropriate ion concentration of Mg. Our findings reveal that the preparation of a lyophilized CS coating is an effective method to promote surface bioactivity and mediate Mg concentration dependent osteogenesis and angiogenesis, which have great potential in bone regeneration.
骨缺损修复在骨科领域面临重大挑战，因此对骨替代物的需求不断增加。磷酸镁水泥（MPCs）因其优异的力学性能和生物降解性而被广泛用于骨缺损修复。然而，高结晶度和镁离子（Mg）释放不受控制限制了MPCs在骨再生中的表面生物活性。在此，我们通过冻干法制备硫酸软骨素（CS）作为表面涂层，即CMPC。我们发现CS涂层均匀分布，并通过阴离子静电吸附提高了MPC的力学性能，同时介导与降解相关的Mg离子的可控释放。通过体外和体内分析相结合，我们表明CS涂层在增加MC3T3-E1s细胞黏附面积的同时保持了细胞相容性。此外，我们展示了CMPC加速的成骨和血管生成，这与适当的Mg离子浓度有关。我们的研究结果表明，制备冻干CS涂层是促进表面生物活性和介导Mg浓度依赖性成骨和血管生成的有效方法，在骨再生中具有巨大潜力。
