Uttam Isha, Sudarsan Sujesh, Ray Rohitraj, Chinnappan Raja, Yaqinuddin Ahmed, Al-Kattan Khaled, Mani Naresh Kumar
Microfluidics, Sensors and Diagnostics (µSenD) Laboratory, Centre for Microfluidics, Biomarkers, Photoceutics and Sensors (μBioPS), Department of Biotechnology, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal 576104, Karnataka, India.
Department of BioSystems Science and Engineering (BSSE), Indian Institute of Science, CV Raman Rd, Bangalore 560012, Karnataka, India.
Life (Basel). 2023 Dec 25;14(1):38. doi: 10.3390/life14010038.
This hypothesis demonstrates that the efficiency of loop-mediated isothermal amplification (LAMP) for nucleic acid detection can be positively influenced by the preconcentration of microbial cells onto hydrophobic paper surfaces. The mechanism of this model is based on the high affinity of microbes towards hydrophobic surfaces. Extensive studies have demonstrated that hydrophobic surfaces exhibit enhanced bacterial and fungal adhesion. By exploiting this inherent affinity of hydrophobic paper substrates, the preconcentration approach enables the adherence of a greater number of target cells, resulting in a higher concentration of target templates for amplification directly from urine samples. In contrast to conventional methods, which often involve complex procedures, this approach offers a simpler, cost-effective, and user-friendly alternative. Moreover, the integration of cell adhesion, LAMP amplification, and signal readout within paper origami-based devices can provide a portable, robust, and highly efficient platform for rapid nucleic acid detection. This innovative hypothesis holds significant potential for point-of-care (POC) diagnostics and field surveillance applications. Further research and development in this field will advance the implementation of this technology, contributing to improved healthcare systems and public health outcomes.
该假设表明，微生物细胞在疏水纸表面上的预浓缩可对环介导等温扩增（LAMP）核酸检测的效率产生积极影响。该模型的机制基于微生物对疏水表面的高亲和力。大量研究表明，疏水表面表现出增强的细菌和真菌附着力。通过利用疏水纸底物的这种固有亲和力，预浓缩方法能够使更多数量的靶细胞附着，从而直接从尿液样本中获得更高浓度的用于扩增的靶模板。与通常涉及复杂程序的传统方法相比，该方法提供了一种更简单、经济高效且用户友好的替代方案。此外，基于折纸的装置内细胞粘附、LAMP扩增和信号读出的整合可为快速核酸检测提供一个便携、稳健且高效的平台。这一创新假设在即时检测（POC）诊断和现场监测应用中具有巨大潜力。该领域的进一步研发将推动这项技术的实施，有助于改善医疗保健系统和公共卫生成果。
