Nikol'skaia I I, Anikeĭcheva N V, Aleksandrova S S, Debov S S
Vopr Med Khim. 1979 Jul-Aug;25(4):395-7.
Superspiralized coiled plasmide DNA were found in cells E. coli CK. One of plasmides pEco CK-1 had a size 21.2 micron that corresponded to molecular mass 43.7 megadaltons, another plasmide pEco CK-2 with the size 14.2 micron had a molecular weight 29.2 megadaltons. Effective restriction was observed in cross titration using phage PBV-I.CK for E. coli strains carrying R II plasmide and phage PBV-I.R II for E. coli CK cells. The data obtained suggest that the Eco CK type with DNA of host specificity is distinct from hsp II type due to presence of R II and N-15 plasmides.
在大肠杆菌CK细胞中发现了超螺旋化的环状质粒DNA。其中一个质粒pEco CK - 1大小为21.2微米,对应分子量为43.7兆道尔顿,另一个大小为14.2微米的质粒pEco CK - 2分子量为29.2兆道尔顿。在用噬菌体PBV - I.CK对携带R II质粒的大肠杆菌菌株以及用噬菌体PBV - I.R II对大肠杆菌CK细胞进行交叉滴定中观察到了有效的限制作用。所获得的数据表明,由于存在R II和N - 15质粒,具有宿主特异性DNA的Eco CK型与hsp II型不同。