School of Chemical Engineering&Technology, China University of Mining and Technology, Xuzhou, 221100, China; Key Laboratory of Spin Electron and Nanomaterials of Anhui Higher Education Institutes, School of Chemistry and Chemical Engineering, Suzhou University, Suzhou, 234000, China.
School of Chemical Engineering&Technology, China University of Mining and Technology, Xuzhou, 221100, China.
Anal Chim Acta. 2024 Feb 22;1291:342240. doi: 10.1016/j.aca.2024.342240. Epub 2024 Jan 11.
Cancer posed a serious threat to human health, and early diagnosis of cancer biomarker was extremely important for the treatment and control of cancer. Electrochemistry-based assays were low-cost, responsive and easy to operate, but there were some challenges in terms of accuracy, detection limit, efficiency and portability. The combination of microfluidic devices and electrochemical methods was expected to construct a high-performance sensing platform, but long-time antigen-antibody incubation was still required. Therefore, a novel microfluidic chip needs to be developed, which has the advantages of good portability, short incubation time, high accuracy, low detection limit and great application to point-of-care testing.
A microfluidic sensor based on microcolumn array electrodes was developed, in which microcolumns could create local mixed flow to reduce the incubation time of target molecules and enhance their interaction with the sensing interface. Besides, three dimensional Mxene fibers-gold nanoparticles (3D MF-Au) was modified on the microcolumn array electrodes to increase active sites and provide more electrolyte shuttle holes. The electrolyte turbulence caused by the microcolumn array electrodes could heighten the contact between the target molecules and sensing interface and accelerate the transfer of redox pairs, thus reducing the incubation time of the target molecules and improving the electrochemical responses in synergy with the 3D MF-Au. Herein, the detection of AFP was chosen as a model, and the microfluidic sensor possessed superior performance for analysis of AFP in the range of 0.1 pg mL - 200 ng mL with a low detection limit (LOD) of 0.0648 pg mL.
This microfluidic chip integrating with microcolumn array electrodes has been successfully implemented to detect AFP in human serum, and the results were consistent with that of electrochemical chemiluminescence method. The microfluidic chip provided a new strategy of portability, shortening incubation time and enhancing electrical signals for antigen detection of real samples, which showed great utilization potentiality in point-of-care testing.
癌症严重威胁人类健康，癌症生物标志物的早期诊断对癌症的治疗和控制至关重要。基于电化学的分析方法具有成本低、响应快、易于操作等优点，但在准确性、检测限、效率和便携性方面仍存在一些挑战。将微流控器件与电化学方法相结合，有望构建高性能传感平台，但仍需要长时间的抗原-抗体孵育。因此，需要开发一种新型微流控芯片，该芯片具有良好的便携性、短的孵育时间、高的准确性、低的检测限和对即时检测的巨大应用潜力。
开发了一种基于微柱阵列电极的微流控传感器，其中微柱可以产生局部混合流，从而缩短目标分子的孵育时间并增强它们与传感界面的相互作用。此外，在微柱阵列电极上修饰了三维 MXene 纤维-金纳米粒子（3D MF-Au），以增加活性位点并提供更多的电解质穿梭孔。微柱阵列电极引起的电解质紊流可以提高目标分子与传感界面的接触，并加速电子对的转移，从而协同 3D MF-Au 缩短目标分子的孵育时间并提高电化学响应。在此，选择 AFP 的检测作为模型，该微流控传感器在 0.1 pg mL-200 ng mL 的范围内对 AFP 的分析具有优异的性能，检测限（LOD）低至 0.0648 pg mL。
该微流控芯片与微柱阵列电极集成，成功用于检测人血清中的 AFP，结果与电化学化学发光法一致。该微流控芯片为抗原检测提供了便携性、缩短孵育时间和增强电信号的新策略，在即时检测方面具有巨大的应用潜力。
